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@ARTICLE{nnep:888342,
author = {Ünnep, Renáta and Paul, Suman and Zsiros, Ottó and
Kovács, László and Székely, Noémi K. and Steinbach,
Gábor and Appavou, Marie-Sousai and Porcar, Lionel and
Holzwarth, Alfred R. and Garab, Győző and Nagy, Gergely},
title = {{T}hylakoid membrane reorganizations revealed by
small-angle neutron scattering of {M}onstera deliciosa
leaves associated with non-photochemical quenching},
journal = {Open biology},
volume = {10},
number = {9},
issn = {2046-2441},
address = {London},
publisher = {Royal Society Publishing},
reportid = {FZJ-2020-04860},
pages = {200144 -},
year = {2020},
abstract = {Non-photochemical quenching (NPQ) is an important
photoprotective mechanism in plants and algae. Although the
process is extensively studied, little is known about its
relationship with ultrastructural changes of the thylakoid
membranes. In order to better understand this relationship,
we studied the effects of illumination on the organization
of thylakoid membranes in Monstera deliciosa leaves. This
evergreen species is known to exhibit very large NPQ and to
possess giant grana with dozens of stacked thylakoids. It is
thus ideally suited for small-angle neutron scattering
measurements (SANS)—a non-invasive technique, which is
capable of providing spatially and statistically averaged
information on the periodicity of the thylakoid membranes
and their rapid reorganizations in vivo. We show that
NPQ-inducing illumination causes a strong decrease in the
periodic order of granum thylakoid membranes. Development of
NPQ and light-induced ultrastructural changes, as well as
the relaxation processes, follow similar kinetic patterns.
Surprisingly, whereas NPQ is suppressed by diuron, it
impedes only the relaxation of the structural changes and
not its formation, suggesting that structural changes do not
cause but enable NPQ. We also demonstrate that the
diminishment of SANS peak does not originate from
light-induced redistribution and reorientation of
chloroplasts inside the cells.},
cin = {JCNS-FRM-II / JCNS-1 / MLZ},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106 / I:(DE-588b)4597118-3},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {32931722},
UT = {WOS:000574886200004},
doi = {10.1098/rsob.200144},
url = {https://juser.fz-juelich.de/record/888342},
}