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000888343 1001_ $$0P:(DE-HGF)0$$ade Santis, Augusta$$b0
000888343 245__ $$aNot just a fluidifying effect: omega-3 phospholipids induce formation of non-lamellar structures in biomembranes
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000888343 520__ $$aPolyunsaturated omega-3 fatty acid docosahexaenoic acid (DHA) is found in very high concentrations in a few peculiar tissues, suggesting that it must have a specialized role. DHA was proposed to affect the function of the cell membrane and related proteins through an indirect mechanism of action, based on the DHA-phospholipid effects on the lipid bilayer structure. In this respect, most studies have focused on its influence on lipid-rafts, somehow neglecting the analysis of effects on liquid disordered phases that constitute most of the cell membranes, by reporting in these cases only a general fluidifying effect. In this study, by combining neutron reflectivity, cryo-transmission electron microscopy, small angle neutron scattering, dynamic light scattering and electron paramagnetic resonance spectroscopy, we characterize liquid disordered bilayers formed by the naturally abundant 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and different contents of a di-DHA glycero-phosphocholine, 22:6-22:6PC, from both a molecular/microscopic and supramolecular/mesoscopic viewpoint. We show that, below a threshold concentration of about 40% molar percent, incorporation of 22:6-22:6PC in the membrane increases the lipid dynamics slightly but sufficiently to promote the membrane deformation and increase of multilamellarity. Notably, beyond this threshold, 22:6-22:6PC disfavours the formation of lamellar phases, leading to a phase separation consisting mostly of small spherical particles that coexist with a minority portion of a lipid blob with water-filled cavities. Concurrently, from a molecular viewpoint, the polyunsaturated acyl chains tend to fold and expose the termini to the aqueous medium. We propose that this peculiar tendency is a key feature of the DHA-phospholipids making them able to modulate the local morphology of biomembranes.
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000888343 7001_ $$00000-0003-3389-6942$$aVitiello, Giuseppe$$b1
000888343 7001_ $$0P:(DE-Juel1)130507$$aAppavou, Marie-Sousai$$b2
000888343 7001_ $$00000-0002-6390-052X$$aScoppola, Ernesto$$b3
000888343 7001_ $$0P:(DE-HGF)0$$aFragneto, Giovanna$$b4
000888343 7001_ $$0P:(DE-Juel1)172014$$aBarnsley, Lester C.$$b5
000888343 7001_ $$0P:(DE-HGF)0$$aClifton, Luke A.$$b6
000888343 7001_ $$00000-0002-4681-4718$$aOttaviani, Maria Francesca$$b7
000888343 7001_ $$0P:(DE-HGF)0$$aPaduano, Luigi$$b8
000888343 7001_ $$00000-0001-8124-6034$$aRusso Krauss, Irene$$b9$$eCorresponding author
000888343 7001_ $$00000-0001-6383-8618$$aD’Errico, Gerardino$$b10$$eCorresponding author
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