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@ARTICLE{Pietschmann:888360,
      author       = {Pietschmann, Jan and Dittmann, Dominik and Spiegel, Holger
                      and Krause, Hans-Joachim and Schröper, Florian},
      title        = {{A} {N}ovel {M}ethod for {A}ntibiotic {D}etection in {M}ilk
                      {B}ased on {C}ompetitive {M}agnetic {I}mmunodetection},
      journal      = {Foods},
      volume       = {9},
      number       = {12},
      issn         = {2304-8158},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2020-04866},
      pages        = {1773 -},
      year         = {2020},
      abstract     = {The misuse of antibiotics as well as incorrect dosage or
                      insufficient time for detoxification can result in the
                      presence of pharmacologically active molecules in fresh
                      milk. Hence, in many countries, commercially available milk
                      has to be tested with immunological, chromatographic or
                      microbiological analytical methods to avoid consumption of
                      antibiotic residues. Here a novel, sensitive and portable
                      assay setup for the detection and quantification of
                      penicillin and kanamycin in whole fat milk (WFM) based on
                      competitive magnetic immunodetection (cMID) is described and
                      assay accuracy determined. For this, penicillin G and
                      kanamycin-conjugates were generated and coated onto a matrix
                      of immunofiltration columns (IFC). Biotinylated penicillin G
                      or kanamycin-specific antibodies were pre-incubated with
                      antibiotics-containing samples and subsequently applied onto
                      IFC to determine the concentration of antibiotics through
                      the competition of antibody-binding to the
                      antibiotic-conjugate molecules. Bound antibodies were
                      labeled with streptavidin-coated magnetic particles and
                      quantified using frequency magnetic mixing technology. Based
                      on calibration measurements in WFM with detection limits of
                      1.33 ng·mL−1 for penicillin G and 1.0 ng·mL−1 for
                      kanamycin, spiked WFM samples were analyzed, revealing
                      highly accurate recovery rates and assay precision. Our
                      results demonstrate the suitability of cMID-based
                      competition assay for reliable and easy on-site testing of
                      milk.},
      cin          = {IBI-3},
      ddc          = {640},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33265942},
      UT           = {WOS:000602125800001},
      doi          = {10.3390/foods9121773},
      url          = {https://juser.fz-juelich.de/record/888360},
}