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@ARTICLE{FigueroaMiranda:888407,
      author       = {Figueroa-Miranda, Gabriela and Wu, Changtong and Zhang,
                      Yuting and Nörbel, Lena and Lo, Young and Tanner, Julian
                      Alexander and Elling, Lothar and Offenhäusser, Andreas and
                      Mayer, Dirk},
      title        = {{P}olyethylene glycol-mediated blocking and monolayer
                      morphology of an electrochemical aptasensor for malaria
                      biomarker detection in human serum},
      journal      = {Bioelectrochemistry},
      volume       = {136},
      issn         = {1567-5394},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2020-04884},
      pages        = {107589 -},
      year         = {2020},
      abstract     = {Better approaches are critically needed for in situ
                      point-of-care diagnostic biosensors that enable primary care
                      physicians, or even individual patients, to directly analyze
                      biological fluids without complicated sample pretreatments.
                      Additional purification steps consume time, consume
                      reagents, often require other equipment, and can introduce
                      false-negative results. Biosensors have been modified with
                      blocking molecules to reduce biofouling; however, the
                      effectiveness relies on their chemical composition and
                      morphology. Here, we used a polyethylene glycol film to
                      suppress unspecific binding from human serum on an
                      electrochemical malaria aptasensor. A detailed study of the
                      variation of the chemical and morphological composition of
                      the aptamer/polyethylene glycol mixed monolayer as a
                      function of incubation time was conducted. Higher resistance
                      to matrix biofouling was found for polyethylene glycol than
                      for hydrophobic alkanethiol films. The best sensor
                      performance was observed for intermediate polyethylene
                      glycol immobilization times. With prolonged incubation,
                      phase separation of aptamer, and polyethylene glycol
                      molecules locally increased the aptamer density and thereby
                      diminished the analyte binding capability. Remarkably,
                      polyethylene glycols do not affect the aptasensor
                      sensitivity but enhance the complex matrix tolerance, the
                      dynamic range, and the limit of detection. Careful tuning of
                      the blocking molecule immobilization is crucial to achieving
                      high aptasensor performance and biofouling resistance.},
      cin          = {IBI-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32679336},
      UT           = {WOS:000579732600013},
      doi          = {10.1016/j.bioelechem.2020.107589},
      url          = {https://juser.fz-juelich.de/record/888407},
}