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@ARTICLE{Bosze:888469,
author = {Bosze, Bernadett and Ono, Yosuke and Mattes, Benjamin and
Sinner, Claude and Gourain, Victor and Thumberger, Thomas
and Tlili, Sham and Wittbrodt, Joachim and Saunders, Timothy
E. and Strähle, Uwe and Schug, Alexander and Scholpp,
Steffen},
title = {{P}cdh18a regulates endocytosis of {E}-cadherin during
axial mesoderm development in zebrafish},
journal = {Histochemistry and cell biology},
volume = {154},
number = {5},
issn = {0948-6143},
address = {Berlin},
publisher = {Springer70296},
reportid = {FZJ-2020-04937},
pages = {463-480},
year = {2020},
abstract = {The notochord defines the axial structure of all
vertebrates during development. Notogenesis is a result of
major cell reorganization in the mesoderm, the convergence
and the extension of the axial cells. However, it is
currently not fully understood how these processes act
together in a coordinated way during notochord formation.
The prechordal plate is an actively migrating cell
population in the central mesoderm anterior to the trailing
notochordal plate cells. We show that prechordal plate cells
express Protocadherin 18a (Pcdh18a), a member of the
cadherin superfamily. We find that Pcdh18a-mediated
recycling of E-cadherin adhesion complexes transforms
prechordal plate cells into a cohesive and fast migrating
cell group. In turn, the prechordal plate cells subsequently
instruct the trailing mesoderm. We simulated cell migration
during early mesoderm formation using a lattice-based
mathematical framework and predicted that the requirement
for an anterior, local motile cell cluster could guide the
intercalation and extension of the posterior, axial cells.
Indeed, a grafting experiment validated the prediction and
local Pcdh18a expression induced an ectopic prechordal
plate-like cell group migrating towards the animal pole. Our
findings indicate that the Pcdh18a is important for
prechordal plate formation, which influences the trailing
mesodermal cell sheet by orchestrating the morphogenesis of
the notochord.},
cin = {JSC / NIC},
ddc = {570},
cid = {I:(DE-Juel1)JSC-20090406 / I:(DE-Juel1)NIC-20090406},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511) / Forschergruppe Schug $(hkf6_20200501)$},
pid = {G:(DE-HGF)POF3-511 / $G:(DE-Juel1)hkf6_20200501$},
typ = {PUB:(DE-HGF)16},
pubmed = {32488346},
UT = {WOS:000537401300001},
doi = {10.1007/s00418-020-01887-5},
url = {https://juser.fz-juelich.de/record/888469},
}