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000888471 005__ 20210130010945.0
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000888471 037__ $$aFZJ-2020-04939
000888471 041__ $$aEnglish
000888471 1001_ $$0P:(DE-Juel1)138266$$aSchrader, Tobias Erich$$b0$$eCorresponding author$$ufzj
000888471 1112_ $$aJoint Polish-German Crystallographic Meeting 2020$$cBreslau$$d2020-02-24 - 2020-02-27$$wPoland
000888471 245__ $$aNeutron protein crystallography at theHeinz Maier-Leibnitz Zentrum (MLZ)New developments and recent application examples
000888471 260__ $$c2020
000888471 3367_ $$033$$2EndNote$$aConference Paper
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000888471 520__ $$aThe neutron single crystal diffractometer BIODIFF at the research reactor Heinz Maier-Leibnitz (FRM II) is especially designed to collect data from crystals with large unit cells. The main field of application is the structural analysis of proteins, especially the determination of hydrogen atom positions. BIODIFF is a joint project of the Jülich Centre for Neutron Science (JCNS) and the FRM II. BIODIFF is designed as a monochromatic instrument with a narrow wavelength spread of less than 3 %. To cover a large solid angle the main detector of BIODIFF consists of a neutron imaging plate in a cylindrical geometry with online read-out capability. BIODFF is equipped with a standard Oxford Cryosystem “Cryostream 700+” which allows measurements at 100 K. A new kappa goniometer head was added recently. This allows an automated tilting of the crystal in order to increase the completeness of the data set when recording another set of frames in the tilted geometry. Typical scientific questions addressed are the determination of protonation states of amino acid side chains in proteins and the characterization of the hydrogen bonding networks between the protein active centre and an inhibitor or substrate. Picking out some recent highlights from measurements at BIODIFF it will be shown how the method of neutron protein crystallography could be used to answer mechanistic questions in enzymatic processes or help to improve inhibitor fragment screening. New developments at the instrument will also be presented: A new collimation for the primary beam should lead to a reduction in background. It should also make it easier to align the neutron beam with the centre of the neutron imaging plate detector.
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000888471 693__ $$0EXP:(DE-MLZ)BIODIFF-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)BIODIFF-20140101$$6EXP:(DE-MLZ)NL1-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz $$eBIODIFF: Diffractometer for large unit cells$$fNL1$$x0
000888471 7001_ $$0P:(DE-HGF)0$$aOstermann, Andreas$$b1
000888471 8564_ $$uhttps://juser.fz-juelich.de/record/888471/files/talk_SchraderTobiasErichcut.pdf$$yOpenAccess
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000888471 9141_ $$y2020
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