% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Minopoli:888501,
      author       = {Minopoli, Antonio and Della Ventura, Bartolomeo and Lenyk,
                      Bohdan and Gentile, Francesco and Tanner, Julian A. and
                      Offenhäusser, Andreas and Mayer, Dirk and Velotta,
                      Raffaele},
      title        = {{U}ltrasensitive antibody-aptamer plasmonic biosensor for
                      malaria biomarker detection in whole blood},
      journal      = {Nature Communications},
      volume       = {11},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {FZJ-2020-04965},
      pages        = {6134},
      year         = {2020},
      abstract     = {Development of plasmonic biosensors combining reliability
                      and ease of use is still a challenge. Gold nanoparticle
                      arrays made by block copolymer micelle nanolithography
                      (BCMN) stand out for their scalability, cost-effectiveness
                      and tunable plasmonic properties, making them ideal
                      substrates for fluorescence enhancement. Here, we describe a
                      plasmon-enhanced fluorescence immunosensor for the specific
                      and ultrasensitive detection of Plasmodium falciparum
                      lactate dehydrogenase (PfLDH)—a malaria marker—in whole
                      blood. Analyte recognition is realized by oriented
                      antibodies immobilized in a close-packed configuration via
                      the photochemical immobilization technique (PIT), with a top
                      bioreceptor of nucleic acid aptamers recognizing a different
                      surface of PfLDH in a sandwich conformation. The combination
                      of BCMN and PIT enabled maximum control over the
                      nanoparticle size and lattice constant as well as the
                      distance of the fluorophore from the sensing surface. The
                      device achieved a limit of detection smaller than 1 pg/mL
                      (<30 fM) with very high specificity without any sample
                      pretreatment. This limit of detection is several orders of
                      magnitude lower than that found in malaria rapid diagnostic
                      tests or even commercial ELISA kits. Thanks to its overall
                      dimensions, ease of use and high-throughput analysis, the
                      device can be used as a substrate in automated multi-well
                      plate readers and improve the efficiency of conventional
                      fluorescence immunoassays.},
      cin          = {IBI-3},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33262332},
      UT           = {WOS:000598899800001},
      doi          = {10.1038/s41467-020-19755-0},
      url          = {https://juser.fz-juelich.de/record/888501},
}