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@ARTICLE{Wu:888505,
author = {Wu, Changtong and Offenhäusser, Andreas and Mayer, Dirk},
title = {{A} {H}ighly {S}ensitive {A}mperometric {A}ptamer
{B}iosensor for {A}denosine {T}riphosphate {D}etection on a
64 {C}hannel {G}old {M}ultielectrode {A}rray},
journal = {Physica status solidi / A},
volume = {217},
number = {13},
issn = {1862-6319},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {FZJ-2020-04969},
pages = {1900925 -},
year = {2020},
abstract = {Herein, an electrochemical aptamer biosensor based on a
gold multielectrode array is reported for the detection of
adenosine triphosphate (ATP), which is an important
neurotransmitter in the central nervous system. The
aptasensor possesses 64 individually addressable
microelectrodes with a diameter of 24 μm, which is
distributed over an area of 1 mm2. The electrodes are
modified with an ATP‐specific aptamer, which is labeled
with a ferrocene redox probe. It is demonstrated that the
used chip fabrication and cleaning process generate
microelectrode channels with similar electrochemical
responses and electroactive surface areas. The
immobilization of the aptamer on gold microelectrodes is
optimized and the receptor density is determined to be
(1.0 ± 0.44) × 1013 molecules cm−2.
Concentration‐dependent alternating current voltammetry
measurements exhibit a limit of detection of
0.30 × 10−9 m and a sensitivity of 2.4 nA logC.
Furthermore, the sensor shows a high selectivity over
analogous molecules and reproducible detection results in
artificial cerebrospinal fluid. The sensor array is used to
detect ATP via several channels of the same chip, showing
high signal redundancy. A label‐free electrochemical,
multichannel ATP aptasensor is demonstrated with high
selectivity and subnanomolar sensitivity, which may be
conducive to record this neurotransmitter in neuronal
cell‐culture systems.},
cin = {IBI-3},
ddc = {530},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
pid = {G:(DE-HGF)POF3-523},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000525922800001},
doi = {10.1002/pssa.201900925},
url = {https://juser.fz-juelich.de/record/888505},
}