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@ARTICLE{Li:888536,
author = {Li, Yuan and Mohanty, Sandipan and Nilsson, Daniel and
Hansson, Bengt and Mao, Kangshan and Irbäck, Anders},
title = {{W}hen a foreign gene meets its native counterpart:
computational biophysics analysis of two {P}gi{C} loci in
the grass {F}estuca ovina},
journal = {Scientific reports},
volume = {10},
number = {1},
issn = {2045-2322},
address = {London},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2020-05000},
pages = {18752},
year = {2020},
abstract = {Duplicative horizontal gene transfer may bring two
previously separated homologous genes together, which may
raise questions about the interplay between the gene
products. One such gene pair is the “native” PgiC1 and
“foreign” PgiC2 in the perennial grass Festuca ovina.
Both PgiC1 and PgiC2 encode cytosolic phosphoglucose
isomerase, a dimeric enzyme whose proper binding is
functionally essential. Here, we use biophysical simulations
to explore the inter-monomer binding of the two homodimers
and the heterodimer that can be produced by PgiC1 and PgiC2
in F. ovina. Using simulated native-state ensembles, we
examine the structural properties and binding tightness of
the dimers. In addition, we investigate their ability to
withstand dissociation when pulled by a force. Our results
suggest that the inter-monomer binding is tighter in the
PgiC2 than the PgiC1 homodimer, which could explain the more
frequent occurrence of the foreign PgiC2 homodimer in dry
habitats. We further find that the PgiC1 and PgiC2 monomers
are compatible with heterodimer formation; the computed
binding tightness is comparable to that of the PgiC1
homodimer. Enhanced homodimer stability and capability of
heterodimer formation with PgiC1 are properties of PgiC2
that may contribute to the retaining of the otherwise
redundant PgiC2 gene.},
cin = {JSC},
ddc = {600},
cid = {I:(DE-Juel1)JSC-20090406},
pnm = {511 - Computational Science and Mathematical Methods
(POF3-511)},
pid = {G:(DE-HGF)POF3-511},
typ = {PUB:(DE-HGF)16},
pubmed = {33127989},
UT = {WOS:000591917600027},
doi = {10.1038/s41598-020-75650-0},
url = {https://juser.fz-juelich.de/record/888536},
}