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@ARTICLE{Blenau:888640,
      author       = {Blenau, Wolfgang and Wilms, Joana Alessandra and Balfanz,
                      Sabine and Baumann, Arnd},
      title        = {{A}m{O}ctα2{R}: {F}unctional {C}haracterization of a
                      {H}oneybee {O}ctopamine {R}eceptor {I}nhibiting {A}denylyl
                      {C}yclase {A}ctivity},
      journal      = {International journal of molecular sciences},
      volume       = {21},
      number       = {24},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2020-05083},
      pages        = {9334 -},
      year         = {2020},
      abstract     = {The catecholamines norepinephrine and epinephrine are
                      important regulators of vertebrate physiology. Insects such
                      as honeybees do not synthesize these neuroactive substances.
                      Instead, they use the phenolamines tyramine and octopamine
                      for similar physiological functions. These biogenic amines
                      activate specific members of the large protein family of G
                      protein-coupled receptors (GPCRs). Based on molecular and
                      pharmacological data, insect octopamine receptors were
                      classified as either α- or β-adrenergic-like octopamine
                      receptors. Currently, one α- and four β-receptors have
                      been molecularly and pharmacologically characterized in the
                      honeybee. Recently, an α2-adrenergic-like octopamine
                      receptor was identified in Drosophila melanogaster
                      (DmOctα2R). This receptor is activated by octopamine and
                      other biogenic amines and causes a decrease in intracellular
                      cAMP ([cAMP]i). Here, we show that the orthologous receptor
                      of the honeybee (AmOctα2R), phylogenetically groups in a
                      clade closely related to human α2-adrenergic receptors.
                      When heterologously expressed in an eukaryotic cell line,
                      AmOctα2R causes a decrease in [cAMP]i. The receptor
                      displays a pronounced preference for octopamine over
                      tyramine. In contrast to DmOctα2R, the honeybee receptor is
                      not activated by serotonin. Its activity can be blocked
                      efficiently by 5-carboxamidotryptamine and phentolamine. The
                      functional characterization of AmOctα2R now adds a sixth
                      member to this subfamily of monoaminergic receptors in the
                      honeybee and is an important step towards understanding the
                      actions of octopamine in honeybee behavior and physiology},
      cin          = {IBI-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33302363},
      UT           = {WOS:000603500200001},
      doi          = {10.3390/ijms21249334},
      url          = {https://juser.fz-juelich.de/record/888640},
}