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@ARTICLE{Tuber:888712,
      author       = {Täuber, Sarah and Golze, Corinna and Ho, Phuong and von
                      Lieres, Eric and Grünberger, Alexander},
      title        = {d{MSCC}: a microfluidic platform for microbial single-cell
                      cultivation of {C}orynebacterium glutamicum under dynamic
                      environmental medium conditions},
      journal      = {Lab on a chip},
      volume       = {20},
      number       = {23},
      issn         = {1473-0189},
      address      = {Cambridge},
      publisher    = {RSC},
      reportid     = {FZJ-2020-05147},
      pages        = {4442 - 4455},
      year         = {2020},
      abstract     = {In nature and in technical systems, microbial cells are
                      often exposed to rapidly fluctuating environmental
                      conditions. These conditions can vary in quality, e.g., the
                      existence of a starvation zone, and quantity, e.g., the
                      average residence time in this zone. For strain development
                      and process design, cellular response to such fluctuations
                      needs to be systematically analysed. However, the existing
                      methods for physically imitating rapidly changing
                      environmental conditions are limited in spatio-temporal
                      resolution. Hence, we present a novel microfluidic system
                      for cultivation of single cells and small cell clusters
                      under dynamic environmental conditions (dynamic microfluidic
                      single-cell cultivation (dMSCC)). This system enables the
                      control of nutrient availability and composition between two
                      media with second to minute resolution. We validate our
                      technology using the industrially relevant model organism
                      Corynebacterium glutamicum. The organism was exposed to
                      different oscillation frequencies between nutrient excess
                      (feasts) and scarcity (famine). The resulting changes in
                      cellular physiology, such as the colony growth rate and cell
                      morphology, were analysed and revealed significant
                      differences in the growth rate and cell length between the
                      different conditions. dMSCC also allows the application of
                      defined but randomly changing nutrient conditions, which is
                      important for reproducing more complex conditions from
                      natural habitats and large-scale bioreactors. The presented
                      system lays the foundation for the cultivation of cells
                      under complex changing environmental conditions.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33095214},
      UT           = {WOS:000592314900007},
      doi          = {10.1039/D0LC00711K},
      url          = {https://juser.fz-juelich.de/record/888712},
}