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000888784 041__ $$aEnglish
000888784 1001_ $$0P:(DE-Juel1)166572$$aNiether, Doreen$$b0$$eCorresponding author
000888784 1112_ $$a2020 EUSMI User Meeting$$cvirtual conference$$d2020-11-19 - 2020-11-19$$wGermany
000888784 245__ $$aThermophoresis: The Case of Streptavidin and Biotin
000888784 260__ $$c2020
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000888784 520__ $$aThermophoretic behavior of a free protein changes upon ligand binding and gives access to information on the binding constants. The Soret effect has also been proven to be a promising tool to gain information on the hydration layer, as the temperature dependence of the thermodiffusion behavior is sensitive to solute-solvent interactions [1]. In this work, we perform systematic thermophoretic measurements of the protein streptavidin (STV) and of the complex STV with biotin (B) using thermal diffusion forced Rayleigh scattering (TDFRS) [2]. Our experiments show that the temperature sensitivity of the Soret coefficient is reduced for the complex compared to the free protein. We discuss our data in comparison with recent quasi-elastic neutron scattering (QENS) measurements. As the QENS measurement has been performed in heavy water, we perform additional measurements in water/heavy water mixtures. Finally, we also elucidate the challenges arising from the quantiative thermophoretic study of complex multicomponent systems such as protein solutions. References[1] “Thermophoresis of biological and biocompatible compounds in aqueous solution”; Niether, D; Wiegand, S.; J. Phys. Condens. Matter, 31, 503003, (2019). DOI: 10.1088/1361-648X/ab421c[2] “Thermophoresis: The Case of Streptavidin and Biotin”; Niether, D; Sarter, M; Koenig, B.W. et al; Polymers, 12, 376, (2020). DOI: 10.3390/polym12020376.
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000888784 7001_ $$0P:(DE-Juel1)171618$$aSarter, Mona$$b1
000888784 7001_ $$0P:(DE-Juel1)132009$$aKönig, Bernd$$b2$$ufzj
000888784 7001_ $$0P:(DE-Juel1)131961$$aFitter, Jörg$$b3$$ufzj
000888784 7001_ $$0P:(DE-Juel1)140278$$aStadler, Andreas$$b4$$ufzj
000888784 7001_ $$0P:(DE-Juel1)131034$$aWiegand, Simone$$b5$$ufzj
000888784 8564_ $$uhttps://juser.fz-juelich.de/record/888784/files/2020-11-19_EUSMI_Thermophoresis_Streptavidin.pdf$$yOpenAccess
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