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@INPROCEEDINGS{Niether:888784,
      author       = {Niether, Doreen and Sarter, Mona and König, Bernd and
                      Fitter, Jörg and Stadler, Andreas and Wiegand, Simone},
      title        = {{T}hermophoresis: {T}he {C}ase of {S}treptavidin and
                      {B}iotin},
      reportid     = {FZJ-2020-05213},
      year         = {2020},
      abstract     = {Thermophoretic behavior of a free protein changes upon
                      ligand binding and gives access to information on the
                      binding constants. The Soret effect has also been proven to
                      be a promising tool to gain information on the hydration
                      layer, as the temperature dependence of the thermodiffusion
                      behavior is sensitive to solute-solvent interactions [1]. In
                      this work, we perform systematic thermophoretic measurements
                      of the protein streptavidin (STV) and of the complex STV
                      with biotin (B) using thermal diffusion forced Rayleigh
                      scattering (TDFRS) [2]. Our experiments show that the
                      temperature sensitivity of the Soret coefficient is reduced
                      for the complex compared to the free protein. We discuss our
                      data in comparison with recent quasi-elastic neutron
                      scattering (QENS) measurements. As the QENS measurement has
                      been performed in heavy water, we perform additional
                      measurements in water/heavy water mixtures. Finally, we also
                      elucidate the challenges arising from the quantiative
                      thermophoretic study of complex multicomponent systems such
                      as protein solutions. References[1] “Thermophoresis of
                      biological and biocompatible compounds in aqueous
                      solution”; Niether, D; Wiegand, S.; J. Phys. Condens.
                      Matter, 31, 503003, (2019). DOI: 10.1088/1361-648X/ab421c[2]
                      “Thermophoresis: The Case of Streptavidin and Biotin”;
                      Niether, D; Sarter, M; Koenig, B.W. et al; Polymers, 12,
                      376, (2020). DOI: 10.3390/polym12020376.},
      month         = {Nov},
      date          = {2020-11-19},
      organization  = {2020 EUSMI User Meeting, virtual
                       conference (Germany), 19 Nov 2020 - 19
                       Nov 2020},
      subtyp        = {Invited},
      cin          = {IBI-4 / IEK-8},
      cid          = {I:(DE-Juel1)IBI-4-20200312 / I:(DE-Juel1)IEK-8-20101013},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/888784},
}