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@ARTICLE{Lee:888811,
      author       = {Lee, Jungho and Hilgers, Fabienne and Loeschke, Anita and
                      Jaeger, Karl-Erich and Feldbrügge, Michael},
      title        = {{U}stilago maydis {S}erves as a {N}ovel {P}roduction {H}ost
                      for the {S}ynthesis of {P}lant and {F}ungal
                      {S}esquiterpenoids},
      journal      = {Frontiers in microbiology},
      volume       = {11},
      issn         = {1664-302X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2020-05225},
      pages        = {1655},
      year         = {2020},
      abstract     = {Sesquiterpenoids are important secondary metabolites with
                      various pharma- and nutraceutical properties. In particular,
                      higher basidiomycetes possess a versatile biosynthetic
                      repertoire for these bioactive compounds. To date, only a
                      few microbial production systems for fungal sesquiterpenoids
                      have been established. Here, we introduce Ustilago maydis as
                      a novel production host. This model fungus is a close
                      relative of higher basidiomycetes. It offers the advantage
                      of metabolic compatibility and potential tolerance for
                      substances toxic to other microorganisms. We successfully
                      implemented a heterologous pathway to produce the carotenoid
                      lycopene that served as a straightforward read-out for
                      precursor pathway engineering. Overexpressing genes encoding
                      enzymes of the mevalonate pathway resulted in increased
                      lycopene levels. Verifying the subcellular localization of
                      the relevant enzymes revealed that initial metabolic
                      reactions might take place in peroxisomes: despite the
                      absence of a canonical peroxisomal targeting sequence,
                      acetyl-CoA C-acetyltransferase Aat1 localized to
                      peroxisomes. By expressing the plant (+)-valencene synthase
                      CnVS and the basidiomycete sesquiterpenoid synthase Cop6, we
                      succeeded in producing (+)-valencene and α-cuprenene,
                      respectively. Importantly, the fungal compound yielded about
                      tenfold higher titers in comparison to the plant substance.
                      This proof of principle demonstrates that U. maydis can
                      serve as promising novel chassis for the production of
                      terpenoids.},
      cin          = {IMET},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IMET-20090612},
      pnm          = {583 - Innovative Synergisms (POF3-583)},
      pid          = {G:(DE-HGF)POF3-583},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32849341},
      UT           = {WOS:000560547000001},
      doi          = {10.3389/fmicb.2020.01655},
      url          = {https://juser.fz-juelich.de/record/888811},
}