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@ARTICLE{Graziadei:888814,
      author       = {Graziadei, Andrea and Gabel, Frank and Kirkpatrick, John
                      and Carlomagno, Teresa},
      title        = {{T}he guide s{RNA} sequence determines the activity level
                      of box {C}/{D} {RNP}s},
      journal      = {eLife},
      volume       = {9},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {FZJ-2020-05228},
      pages        = {e50027},
      year         = {2020},
      abstract     = {2’-O-rRNA methylation, which is essential in eukaryotes
                      and archaea, is catalysed by the Box C/D RNP complex in an
                      RNA-guided manner. Despite the conservation of the
                      methylation sites, the abundance of site-specific
                      modifications shows variability across species and tissues,
                      suggesting that rRNA methylation may provide a means of
                      controlling gene expression. As all Box C/D RNPs are thought
                      to adopt a similar structure, it remains unclear how the
                      methylation efficiency is regulated. Here, we provide the
                      first structural evidence that, in the context of the Box
                      C/D RNP, the affinity of the catalytic module fibrillarin
                      for the substrate–guide helix is dependent on the RNA
                      sequence outside the methylation site, thus providing a
                      mechanism by which both the substrate and guide RNA
                      sequences determine the degree of methylation. To reach this
                      result, we develop an iterative structure-calculation
                      protocol that exploits the power of integrative structural
                      biology to characterize conformational ensembles.},
      cin          = {JCNS-FRM-II / MLZ},
      ddc          = {600},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 / I:(DE-588b)4597118-3},
      pnm          = {6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
                      / 6G15 - FRM II / MLZ (POF3-6G15)},
      pid          = {G:(DE-HGF)POF3-6G4 / G:(DE-HGF)POF3-6G15},
      experiment   = {EXP:(DE-MLZ)KWS1-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32202498},
      UT           = {WOS:000521637000001},
      doi          = {10.7554/eLife.50027},
      url          = {https://juser.fz-juelich.de/record/888814},
}