Hauptseite > Publikationsdatenbank > The guide sRNA sequence determines the activity level of box C/D RNPs > print

001     888814
005     20210130011127.0
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100 1 _ |a Graziadei, Andrea
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245 _ _ |a The guide sRNA sequence determines the activity level of box C/D RNPs
260 _ _ |a Cambridge
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520 _ _ |a 2’-O-rRNA methylation, which is essential in eukaryotes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner. Despite the conservation of the methylation sites, the abundance of site-specific modifications shows variability across species and tissues, suggesting that rRNA methylation may provide a means of controlling gene expression. As all Box C/D RNPs are thought to adopt a similar structure, it remains unclear how the methylation efficiency is regulated. Here, we provide the first structural evidence that, in the context of the Box C/D RNP, the affinity of the catalytic module fibrillarin for the substrate–guide helix is dependent on the RNA sequence outside the methylation site, thus providing a mechanism by which both the substrate and guide RNA sequences determine the degree of methylation. To reach this result, we develop an iterative structure-calculation protocol that exploits the power of integrative structural biology to characterize conformational ensembles.
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693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
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700 1 _ |a Gabel, Frank
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700 1 _ |a Kirkpatrick, John
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700 1 _ |a Carlomagno, Teresa
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773 _ _ |a 10.7554/eLife.50027
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856 4 _ |u https://juser.fz-juelich.de/record/888814/files/elife-50027-v1.pdf
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