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000889023 1001_ $$0P:(DE-HGF)0$$aDi Somma, Angela$$b0
000889023 245__ $$aThe antimicrobial peptide Temporin L impairs E. coli cell division by interacting with FtsZ and the divisome complex
000889023 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2020
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000889023 520__ $$aBackgroundThe comprehension of the mechanism of action of antimicrobial peptides is fundamental for the design of new antibiotics. Studies performed looking at the interaction of peptides with bacterial cells offer a faithful picture of what really happens in nature.MethodsIn this work we focused on the interaction of the peptide Temporin L with E. coli cells, using a variety of biochemical and biophysical techniques that include: functional proteomics, docking, optical microscopy, TEM, DLS, SANS, fluorescence.ResultsWe identified bacterial proteins specifically interacting with the peptides that belong to the divisome machinery; our data suggest that the GTPase FtsZ is the specific peptide target. Docking experiments supported the FtsZ-TL interaction; binding and enzymatic assays using recombinant FtsZ confirmed this hypothesis and revealed a competitive inhibition mechanism. Optical microscopy and TEM measurements demonstrated that, upon incubation with the peptide, bacterial cells are unable to divide forming long necklace-like cell filaments. Dynamic light scattering studies and Small Angle Neutron Scattering experiments performed on treated and untreated bacterial cells, indicated a change at the nanoscale level of the bacterial membrane.ConclusionsThe peptide temporin L acts by a non-membrane-lytic mechanism of action, inhibiting the divisome machinery.
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000889023 7001_ $$0P:(DE-HGF)0$$aAvitabile, Concetta$$b1
000889023 7001_ $$0P:(DE-HGF)0$$aCirillo, Arianna$$b2
000889023 7001_ $$0P:(DE-HGF)0$$aMoretta, Antonio$$b3
000889023 7001_ $$0P:(DE-HGF)0$$aMerlino, Antonello$$b4
000889023 7001_ $$0P:(DE-HGF)0$$aPaduano, Luigi$$b5
000889023 7001_ $$0P:(DE-HGF)0$$aDuilio, Angela$$b6
000889023 7001_ $$0P:(DE-HGF)0$$aRomanelli, Alessandra$$b7$$eCorresponding author
000889023 773__ $$0PERI:(DE-600)2209617-6$$a10.1016/j.bbagen.2020.129606$$gVol. 1864, no. 7, p. 129606 -$$n7$$p129606$$tBiochimica et biophysica acta / General subjects$$v1864$$x0304-4165$$y2020
000889023 8564_ $$uhttps://juser.fz-juelich.de/record/889023/files/LavoroTemporinaL30102019.pdf$$yPublished on 2020-03-27. Available in OpenAccess from 2021-03-27.
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