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| Hauptseite > Publikationsdatenbank > The antimicrobial peptide Temporin L impairs E. coli cell division by interacting with FtsZ and the divisome complex > print |
| Hauptseite > Publikationsdatenbank > The antimicrobial peptide Temporin L impairs E. coli cell division by interacting with FtsZ and the divisome complex > print |
| 001 | 889023 | ||
| 005 | 20210401192809.0 | ||
| 024 | 7 | _ | |a 10.1016/j.bbagen.2020.129606 |2 doi |
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| 245 | _ | _ | |a The antimicrobial peptide Temporin L impairs E. coli cell division by interacting with FtsZ and the divisome complex |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2020 |b Elsevier |
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| 520 | _ | _ | |a BackgroundThe comprehension of the mechanism of action of antimicrobial peptides is fundamental for the design of new antibiotics. Studies performed looking at the interaction of peptides with bacterial cells offer a faithful picture of what really happens in nature.MethodsIn this work we focused on the interaction of the peptide Temporin L with E. coli cells, using a variety of biochemical and biophysical techniques that include: functional proteomics, docking, optical microscopy, TEM, DLS, SANS, fluorescence.ResultsWe identified bacterial proteins specifically interacting with the peptides that belong to the divisome machinery; our data suggest that the GTPase FtsZ is the specific peptide target. Docking experiments supported the FtsZ-TL interaction; binding and enzymatic assays using recombinant FtsZ confirmed this hypothesis and revealed a competitive inhibition mechanism. Optical microscopy and TEM measurements demonstrated that, upon incubation with the peptide, bacterial cells are unable to divide forming long necklace-like cell filaments. Dynamic light scattering studies and Small Angle Neutron Scattering experiments performed on treated and untreated bacterial cells, indicated a change at the nanoscale level of the bacterial membrane.ConclusionsThe peptide temporin L acts by a non-membrane-lytic mechanism of action, inhibiting the divisome machinery. |
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| 773 | _ | _ | |a 10.1016/j.bbagen.2020.129606 |g Vol. 1864, no. 7, p. 129606 - |0 PERI:(DE-600)2209617-6 |n 7 |p 129606 |t Biochimica et biophysica acta / General subjects |v 1864 |y 2020 |x 0304-4165 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/889023/files/LavoroTemporinaL30102019.pdf |y Published on 2020-03-27. Available in OpenAccess from 2021-03-27. |
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