TY  - JOUR
AU  - Becker, Johanna
AU  - Hosseinpour Tehrani, Hamed
AU  - Ernst, Philipp
AU  - Blank, Lars Mathias
AU  - Wierckx, Nick
TI  - An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield
JO  - Journal of Fungi
VL  - 7
IS  - 1
SN  - 2309-608X
CY  - Basel
PB  - MDPI
M1  - FZJ-2021-00007
SP  - 20 -
PY  - 2021
AB  - Ustilago maydis, a member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillusterreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed.
LB  - PUB:(DE-HGF)16
C6  - pmid:33396473
UR  - <Go to ISI:>//WOS:000610334500001
DO  - DOI:10.3390/jof7010020
UR  - https://juser.fz-juelich.de/record/889069
ER  -