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@ARTICLE{Schroeder:889139,
author = {Schroeder, Daniel C. and Popp, Erik and Rohleder, Cathrin
and Vus, Stefanie and Bethencourt, David de la Puente and
Finke, Simon R. and Zlatopolskiy, Boris and Zischler,
Johannes and Drzezga, Alexander and Herff, Holger and
Annecke, Thorsten and Hucho, Tim and Neumaier, Bernd and
Böttiger, Bernd W. and Endepols, Heike},
title = {{P}ositron-{E}mission-{T}omography {I}maging of
{L}ong-{T}erm {E}xpression of the 18k{D}a {T}ranslocator
{P}rotein {A}fter {S}udden {C}ardiac {A}rrest in {R}ats},
journal = {Shock},
volume = {55},
number = {5},
issn = {1073-2322},
address = {Augusta, Ga.},
publisher = {Biomedical Press},
reportid = {FZJ-2021-00064},
pages = {620-629},
year = {2021},
note = {PostPrint liegt leider nicht vor und kann zeitnah auch
nicht eingeholt werden; wird nachgetragen.},
abstract = {Background: Knowledge about the neuroinflammatory state
during months after sudden cardiac arrest is scarce.
Neuroinflammation is mediated by cells that express the
18kDa translocator protein (TSPO). We determined the time
course of TSPO-expressing cells in a rat model of sudden
cardiac arrest using longitudinal in vivo positron emission
tomography (PET) imaging with the TSPO-specific tracer
[F]DAA1106 over a period of 6 months.Methods: Five male
Sprague Dawley rats were resuscitated from 6 minutes sudden
cardiac arrest due to ventricular fibrillation, 3 animals
served as shams. PET measurements were performed on day 5,
8, 14, 90 and 180 after intervention. Magnetic resonance
imaging was performed on day 140. Imaging was preceded by
Barnes Maze spatial memory testing on day 3, 13, 90 and 180.
Specificity of [F]DAA1106 binding was confirmed by Iba-1
immunohistochemistry.Results: [F]DAA1106 accumulated
bilaterally in the dorsal hippocampus of all sudden cardiac
arrest animals on all measured time points.
Immunohistochemistry confirmed Iba-1 expressing cells in the
hippocampal CA1 region. The number of Iba-1-immunoreactive
objects per mm was significantly correlated with [F]DAA1106
uptake. Additionally, two of the five sudden cardiac arrest
rats showed bilateral TSPO-expression in the striatum that
persisted until day 180. In Barnes Maze, the relative time
spent in the target quadrant negatively correlates with
dorsal hippocampal [F]DAA1106 uptake on day 14 and
180.Conclusions: After sudden cardiac arrest, TSPO remains
expressed over the long-term. Sustainable treatment options
for neuroinflammation may be considered to improve cognitive
functions after sudden cardiac arrest.},
cin = {INM-2 / INM-5},
ddc = {610},
cid = {I:(DE-Juel1)INM-2-20090406 / I:(DE-Juel1)INM-5-20090406},
pnm = {5253 - Neuroimaging (POF4-525)},
pid = {G:(DE-HGF)POF4-5253},
typ = {PUB:(DE-HGF)16},
pubmed = {32433203},
UT = {WOS:000663739200008},
doi = {10.1097/SHK.0000000000001546},
url = {https://juser.fz-juelich.de/record/889139},
}