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@ARTICLE{Barnkov:889202,
      author       = {Baránková, Barbora and Lazár, Dušan and Nauš, Jan and
                      Solovchenko, Alexei and Gorelova, Olga and Baulina, Olga and
                      Huber, Gregor and Nedbal, Ladislav},
      title        = {{L}ight absorption and scattering by high light-tolerant,
                      fast-growing {C}hlorella vulgaris {IPPAS} {C}-1 cells},
      journal      = {Algal Research},
      volume       = {49},
      issn         = {2211-9264},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2021-00112},
      pages        = {101881 -},
      year         = {2020},
      abstract     = {Algal cells are highly complex optical systems that can
                      dynamically change their structure. Consequently, absorption
                      and scattering properties of algae change, while the cells
                      are acclimating to different light conditions or during
                      growth and division in a cell cycle. This may be
                      particularly important in algal species that can grow
                      rapidly under very high-light such as Chlorella vulgaris
                      IPPAS C-1 that is studied here. From cell transmittance
                      measured conventionally and using integrating sphere, we
                      evaluated absorption and scattering coefficients and cross
                      sections per cell dry weight and chlorophyll content. This
                      was done for asynchronous cell culture grown in low-light
                      (LL; 220 μmol (photons) m−2 s−1) or high-light (HL;
                      1760 μmol (photons) m−2 s−1) light, as well as
                      during cell cycle of synchronous culture grown in HL. During
                      the cell cycle, we also determined cell ultrastructural
                      organization by transmission electron microscopy, and
                      correlated its parameters with absorption and scattering
                      cross sections per cell dry weight. We found that the IPPAS
                      C-1 cells of asynchronous culture scatter light more than
                      other cells, however, internal organization of the cells
                      that is decisive for scattering is less sensitive to HL and
                      LL treatment than the cell pigment content that controls
                      absorption. The light scattering and absorption were
                      dynamically changed during cell cycle of synchronous cells
                      grown in the HL. Changes in ratio of chloroplast to
                      protoplast area, reflecting amount of scattering chloroplast
                      membrane (outer, inner) interfaces, best correlated with
                      changes in light scattering. We suggest that the increased
                      light scattering by the HL-acclimated IPPAS C-1 cells might
                      be responsible for increased HL resilience reported in the
                      literature. Biotechnological aspect of this study is that
                      the scattering and absorption properties of phytoplankton
                      cells ought to be calibrated for each particular growth
                      phase or irradiance to which the cells are acclimated.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / AF AlgalFertilizer -
                      AlgalFertilizer (20172303)},
      pid          = {G:(DE-HGF)POF4-2171 / G:(BioSC)20172303},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000540736500015},
      doi          = {10.1016/j.algal.2020.101881},
      url          = {https://juser.fz-juelich.de/record/889202},
}