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@ARTICLE{Zinke:889727,
      author       = {Zinke, Maximilian and Sachowsky, Katrin A. A. and Öster,
                      Carl and Zinn-Justin, Sophie and Ravelli, Raimond and
                      Schröder, Gunnar F. and Habeck, Michael and Lange, Adam},
      title        = {{A}rchitecture of the flexible tail tube of bacteriophage
                      {SPP}1},
      journal      = {Nature Communications},
      volume       = {11},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {FZJ-2021-00348},
      pages        = {5759},
      year         = {2020},
      abstract     = {Bacteriophage SPP1 is a double-stranded DNA virus of the
                      Siphoviridae family that infects the bacterium Bacillus
                      subtilis. This family of phages features a long, flexible,
                      non-contractile tail that has been difficult to characterize
                      structurally. Here, we present the atomic structure of the
                      tail tube of phage SPP1. Our hybrid structure is based on
                      the integration of structural restraints from solid-state
                      nuclear magnetic resonance (NMR) and a density map from
                      cryo-EM. We show that the tail tube protein gp17.1 organizes
                      into hexameric rings that are stacked by flexible linker
                      domains and, thus, form a hollow flexible tube with a
                      negatively charged lumen suitable for the transport of DNA.
                      Additionally, we assess the dynamics of the system by
                      combining relaxation measurements with variances in density
                      maps.},
      cin          = {IBI-7},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33188213},
      UT           = {WOS:000594647500010},
      doi          = {10.1038/s41467-020-19611-1},
      url          = {https://juser.fz-juelich.de/record/889727},
}