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@ARTICLE{Marin:889729,
author = {Marin, Egor and Luginina, Aleksandra and Gusach, Anastasiia
and Kovalev, Kirill and Bukhdruker, Sergey and Khorn, Polina
and Polovinkin, Vitaly and Lyapina, Elizaveta and Rogachev,
Andrey and Gordeliy, Valentin and Mishin, Alexey and
Cherezov, Vadim and Borshchevskiy, Valentin},
title = {{S}mall-wedge synchrotron and serial {XFEL} datasets for
{C}ysteinyl leukotriene {GPCR}s},
journal = {Scientific data},
volume = {7},
number = {1},
issn = {2052-4463},
address = {London},
publisher = {Nature Publ. Group},
reportid = {FZJ-2021-00350},
pages = {388},
year = {2020},
abstract = {Structural studies of challenging targets such as G
protein-coupled receptors (GPCRs) have accelerated during
the last several years due to the development of new
approaches, including small-wedge and serial
crystallography. Here, we describe the deposition of seven
datasets consisting of X-ray diffraction images acquired
from lipidic cubic phase (LCP) grown microcrystals of two
human GPCRs, Cysteinyl leukotriene receptors 1 and 2
(CysLT1R and CysLT2R), in complex with various antagonists.
Five datasets were collected using small-wedge synchrotron
crystallography (SWSX) at the European Synchrotron Radiation
Facility with multiple crystals under cryo-conditions. Two
datasets were collected using X-ray free electron laser
(XFEL) serial femtosecond crystallography (SFX) at the Linac
Coherent Light Source, with microcrystals delivered at room
temperature into the beam within LCP matrix by a viscous
media microextrusion injector. All seven datasets have been
deposited in the open-access databases Zenodo and CXIDB.
Here, we describe sample preparation and annotate
crystallization conditions for each partial and full
datasets. We also document full processing pipelines and
provide wrapper scripts for SWSX and SFX data processing.},
cin = {IBI-7},
ddc = {500},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {33184270},
UT = {WOS:000593909300001},
doi = {10.1038/s41597-020-00729-2},
url = {https://juser.fz-juelich.de/record/889729},
}