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| 001 | 889729 | ||
| 005 | 20210208142359.0 | ||
| 024 | 7 | _ | |a 10.1038/s41597-020-00729-2 |2 doi |
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| 100 | 1 | _ | |a Marin, Egor |0 0000-0003-2369-1732 |b 0 |
| 245 | _ | _ | |a Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs |
| 260 | _ | _ | |a London |c 2020 |b Nature Publ. Group |
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| 520 | _ | _ | |a Structural studies of challenging targets such as G protein-coupled receptors (GPCRs) have accelerated during the last several years due to the development of new approaches, including small-wedge and serial crystallography. Here, we describe the deposition of seven datasets consisting of X-ray diffraction images acquired from lipidic cubic phase (LCP) grown microcrystals of two human GPCRs, Cysteinyl leukotriene receptors 1 and 2 (CysLT1R and CysLT2R), in complex with various antagonists. Five datasets were collected using small-wedge synchrotron crystallography (SWSX) at the European Synchrotron Radiation Facility with multiple crystals under cryo-conditions. Two datasets were collected using X-ray free electron laser (XFEL) serial femtosecond crystallography (SFX) at the Linac Coherent Light Source, with microcrystals delivered at room temperature into the beam within LCP matrix by a viscous media microextrusion injector. All seven datasets have been deposited in the open-access databases Zenodo and CXIDB. Here, we describe sample preparation and annotate crystallization conditions for each partial and full datasets. We also document full processing pipelines and provide wrapper scripts for SWSX and SFX data processing. |
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| 700 | 1 | _ | |a Luginina, Aleksandra |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Gusach, Anastasiia |0 0000-0002-2594-8573 |b 2 |
| 700 | 1 | _ | |a Kovalev, Kirill |0 P:(DE-Juel1)169220 |b 3 |
| 700 | 1 | _ | |a Bukhdruker, Sergey |0 0000-0002-0157-532X |b 4 |
| 700 | 1 | _ | |a Khorn, Polina |0 0000-0002-2117-2130 |b 5 |
| 700 | 1 | _ | |a Polovinkin, Vitaly |0 0000-0002-3630-5565 |b 6 |
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| 700 | 1 | _ | |a Rogachev, Andrey |0 P:(DE-HGF)0 |b 8 |
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| 700 | 1 | _ | |a Mishin, Alexey |0 0000-0003-3759-380X |b 10 |
| 700 | 1 | _ | |a Cherezov, Vadim |0 0000-0002-5265-3914 |b 11 |
| 700 | 1 | _ | |a Borshchevskiy, Valentin |0 P:(DE-Juel1)179072 |b 12 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/s41597-020-00729-2 |g Vol. 7, no. 1, p. 388 |0 PERI:(DE-600)2775191-0 |n 1 |p 388 |t Scientific data |v 7 |y 2020 |x 2052-4463 |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/889729/files/Author%20correction_Small-wedge%20synchrotron%20and%20serial%20XFEL%20datasets%20for%20Cysteinyl%20leukotriene%20GPCRs.pdf |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/889729/files/Small-wedge%20synchrotron%20and%20serial%20XFEL%20datasets%20for%20Cysteinyl%20leukotriene%20GPCRs.pdf |
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