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@ARTICLE{Milos:889790,
      author       = {Milos, Frano and Belu, Andreea and Mayer, Dirk and Maybeck,
                      Vanessa and Offenhäusser, Andreas},
      title        = {{P}olymer {N}anopillars {I}nduce {I}ncreased {P}axillin
                      {A}dhesion {A}ssembly and {P}romote {A}xon {G}rowth in
                      {P}rimary {C}ortical {N}eurons},
      journal      = {Advanced biology},
      volume       = {5},
      number       = {2},
      issn         = {2366-7478},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2021-00403},
      pages        = {2000248},
      year         = {2021},
      abstract     = {The complexity of the extracellular matrix consists of
                      micro‐ and nanoscale structures that influence neuronal
                      development through contact guidance. Substrates with
                      defined topographic cues mimic the complex extracellular
                      environment and can improve the interface between cells and
                      biomedical devices as well as potentially serve as tissue
                      engineering scaffolds. This study investigates axon
                      development and growth of primary cortical neurons on
                      OrmoComp nanopillars of various dimensions. Neuronal somas
                      and neurites form adhesions and F‐actin accumulations
                      around the pillars indicating a close contact to the
                      topography. In addition, higher pillars (400 nm) confine the
                      growing neurites, resulting in greater neurite alignment to
                      the topographical pattern compared to lower pillars (100
                      nm). A comprehensive analysis of growth cone dynamics during
                      axon development shows that nanopillars induce earlier axon
                      establishment and change the periodicity of growth cone
                      dynamics by promoting elongation. This results in longer
                      axons compared to the flat substrate. Finally, the increase
                      in surface area available for growth cone coupling provided
                      by nanopillar sidewalls is correlated with increased
                      assembly of paxillin‐rich point contact adhesions and a
                      reduction in actin retrograde flow rates allowing for
                      accelerated and persistent neurite outgrowth.},
      cin          = {IBI-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {524 - Molecular and Cellular Information Processing
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-524},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000672523300008},
      doi          = {10.1002/adbi.202000248},
      url          = {https://juser.fz-juelich.de/record/889790},
}