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@ARTICLE{Caruso:889873,
author = {Caruso, Icaro P. and Guimarães, Giovana C. and Machado,
Vitor B. and Fossey, Marcelo A. and Willbold, Dieter and
Almeida, Fabio C. L. and Souza, Fátima P.},
title = {{B}iophysical and {D}ynamic {C}haracterization of
{F}ine-{T}uned {B}inding of the {H}uman {R}espiratory
{S}yncytial {V}irus {M}2-1 {C}ore {D}omain to {L}ong {RNA}s},
journal = {Journal of virology},
volume = {94},
number = {23},
issn = {1098-5514},
address = {Baltimore, Md.},
publisher = {Soc.},
reportid = {FZJ-2021-00481},
pages = {e01505-20},
year = {2020},
abstract = {The human respiratory syncytial virus (hRSV) M2-1 protein
functions as a processivity and antitermination factor of
the viral polymerase complex. Here, the first evidence that
the hRSV M2-1 core domain (cdM2-1) alone has an unfolding
activity for long RNAs is presented and the biophysical and
dynamic characterization of the cdM2-1/RNA complex is
provided. The main contact region of cdM2-1 with RNA was the
α1-α2-α5-α6 helix bundle, which suffered local
conformational changes and promoted the RNA unfolding
activity. This activity may be triggered by base-pairing
recognition. RNA molecules wrap around the whole cdM2-1,
protruding their termini over the domain. The α2-α3 and
α3-α4 loops of cdM2-1 were marked by an increase in
picosecond internal motions upon RNA binding, even though
they are not directly involved in the interaction. The
results revealed that the cdM2-1/RNA complex originates from
a fine-tuned binding, contributing to the unraveling
interaction aspects necessary for M2-1 activity.},
cin = {IBI-7},
ddc = {610},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
pubmed = {32938771},
UT = {WOS:000595866900011},
doi = {10.1128/JVI.01505-20},
url = {https://juser.fz-juelich.de/record/889873},
}