%0 Journal Article
%A Sanwald, Julia L.
%A Dobner, Jochen
%A Simons, Indra M.
%A Poschmann, Gereon
%A Stühler, Kai
%A Üffing, Alina
%A Hoffmann, Silke
%A Willbold, Dieter
%T Lack of GABARAP-Type Proteins Is Accompanied by Altered Golgi Morphology and Surfaceome Composition
%J International journal of molecular sciences
%V 22
%N 1
%@ 1422-0067
%C Basel
%I Molecular Diversity Preservation International
%M FZJ-2021-00487
%P 85 -
%D 2021
%X GABARAP (γ-aminobutyric acid type A receptor-associated protein) and its paralogues GABARAPL1 and GABARAPL2 comprise a subfamily of autophagy-related Atg8 proteins. They are studied extensively regarding their roles during autophagy. Originally, however, especially GABARAPL2 was discovered to be involved in intra-Golgi transport and homotypic fusion of post-mitotic Golgi fragments. Recently, a broader function of mammalian Atg8s on membrane trafficking through interaction with various soluble N-ethylmaleimide-sensitive factor-attachment protein receptors SNAREs was suggested. By immunostaining and microscopic analysis of the Golgi network, we demonstrate the importance of the presence of individual GABARAP-type proteins on Golgi morphology. Furthermore, triple knockout (TKO) cells lacking the whole GABARAP subfamily showed impaired Golgi-dependent vesicular trafficking as assessed by imaging of fluorescently labelled ceramide. With the Golgi apparatus being central within the secretory pathway, we sought to investigate the role of the GABARAP-type proteins for cell surface protein trafficking. By analysing the surfaceome compositionofTKOs, we identified a subset of cell surface proteins with altered plasma membrane localisation. Taken together, we provide novel insights into an underrated aspect of autophagy-independent functions of the GABARAP subfamily and recommend considering the potential impact of GABARAP subfamily proteins on a plethora of processes during experimental analysis of GABARAP-deficient cells not only in the autophagic context.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 33374830
%U <Go to ISI:>//WOS:000606050100001
%R 10.3390/ijms22010085
%U https://juser.fz-juelich.de/record/889879