TY  - JOUR
AU  - Sanwald, Julia L.
AU  - Dobner, Jochen
AU  - Simons, Indra M.
AU  - Poschmann, Gereon
AU  - Stühler, Kai
AU  - Üffing, Alina
AU  - Hoffmann, Silke
AU  - Willbold, Dieter
TI  - Lack of GABARAP-Type Proteins Is Accompanied by Altered Golgi Morphology and Surfaceome Composition
JO  - International journal of molecular sciences
VL  - 22
IS  - 1
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - FZJ-2021-00487
SP  - 85 -
PY  - 2021
AB  - GABARAP (γ-aminobutyric acid type A receptor-associated protein) and its paralogues GABARAPL1 and GABARAPL2 comprise a subfamily of autophagy-related Atg8 proteins. They are studied extensively regarding their roles during autophagy. Originally, however, especially GABARAPL2 was discovered to be involved in intra-Golgi transport and homotypic fusion of post-mitotic Golgi fragments. Recently, a broader function of mammalian Atg8s on membrane trafficking through interaction with various soluble N-ethylmaleimide-sensitive factor-attachment protein receptors SNAREs was suggested. By immunostaining and microscopic analysis of the Golgi network, we demonstrate the importance of the presence of individual GABARAP-type proteins on Golgi morphology. Furthermore, triple knockout (TKO) cells lacking the whole GABARAP subfamily showed impaired Golgi-dependent vesicular trafficking as assessed by imaging of fluorescently labelled ceramide. With the Golgi apparatus being central within the secretory pathway, we sought to investigate the role of the GABARAP-type proteins for cell surface protein trafficking. By analysing the surfaceome compositionofTKOs, we identified a subset of cell surface proteins with altered plasma membrane localisation. Taken together, we provide novel insights into an underrated aspect of autophagy-independent functions of the GABARAP subfamily and recommend considering the potential impact of GABARAP subfamily proteins on a plethora of processes during experimental analysis of GABARAP-deficient cells not only in the autophagic context.
LB  - PUB:(DE-HGF)16
C6  - 33374830
UR  - <Go to ISI:>//WOS:000606050100001
DO  - DOI:10.3390/ijms22010085
UR  - https://juser.fz-juelich.de/record/889879
ER  -