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@ARTICLE{Soloviov:889941,
author = {Soloviov, Dmytro and Cai, Yong Q. and Bolmatov, Dima and
Suvorov, Alexey and Zhernenkov, Kirill and Zav’yalov,
Dmitry and Bosak, Alexey and Uchiyama, Hiroshi and
Zhernenkov, Mikhail},
title = {{F}unctional lipid pairs as building blocks of
phase-separated membranes},
journal = {Proceedings of the National Academy of Sciences of the
United States of America},
volume = {117},
number = {9},
issn = {1091-6490},
address = {Washington, DC},
publisher = {National Acad. of Sciences},
reportid = {FZJ-2021-00547},
pages = {4749 - 4757},
year = {2020},
abstract = {Biological membranes exhibit a great deal of compositional
and phase heterogeneity due to hundreds of chemically
distinct components. As a result, phase separation processes
in cell membranes are extremely difficult to study,
especially at the molecular level. It is currently believed
that the lateral membrane heterogeneity and the formation of
domains, or rafts, are driven by lipid–lipid and
lipid–protein interactions. Nevertheless, the underlying
mechanisms regulating membrane heterogeneity remain poorly
understood. In the present work, we combine inelastic X-ray
scattering with molecular dynamics simulations to provide
direct evidence for the existence of strongly coupled
transient lipid pairs. These lipid pairs manifest themselves
experimentally through optical vibrational (a.k.a. phononic)
modes observed in binary
(1,2-dipalmitoyl-sn-glycero-3-phosphocholine
[DPPC]–cholesterol) and ternary
(DPPC–1,2-dioleoyl-sn-glycero-3-phosphocholine/1-palmitoyl-2-oleoyl-glycero-3-phosphocholine
[DOPC/POPC]–cholesterol) systems. The existence of a
phononic gap in these vibrational modes is a direct result
of the finite size of patches formed by these lipid pairs.
The observation of lipid pairs provides a spatial
(subnanometer) and temporal (subnanosecond) window into the
lipid–lipid interactions in complex mixtures of
saturated/unsaturated lipids and cholesterol. Our findings
represent a step toward understanding the lateral
organization and dynamics of membrane domains using a
well-validated probe with a high spatial and temporal
resolution.},
cin = {JCNS-FRM-II / JCNS-4 / MLZ / JCNS-2},
ddc = {500},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-4-20201012 / I:(DE-588b)4597118-3 /
I:(DE-Juel1)JCNS-2-20110106},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
/ 6G15 - FRM II / MLZ (POF3-6G15)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G4 /
G:(DE-HGF)POF3-6G15},
experiment = {EXP:(DE-MLZ)MARIA-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {32071249},
UT = {WOS:000518473500048},
doi = {10.1073/pnas.1919264117},
url = {https://juser.fz-juelich.de/record/889941},
}