TY  - JOUR
AU  - Hilgers, Fabienne
AU  - Habash, Samer S.
AU  - Loeschcke, Anita
AU  - Ackermann, Yannic
AU  - Neumann, Stefan
AU  - Heck, Achim
AU  - Klaus, Oliver
AU  - Hage-Hülsmann, Jennifer
AU  - Grundler, Florian M. W.
AU  - Jaeger, Karl-Erich
AU  - Sylvia S. Schleker, A.
AU  - Drepper, Thomas
TI  - Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi
JO  - Microorganisms
VL  - 9
IS  - 1
SN  - 2076-2607
CY  - Basel
PB  - MDPI
M1  - FZJ-2021-01004
SP  - 168 -
PY  - 2021
AB  - Terpenoids constitute one of the largest and most diverse groups within the class of secondary metabolites, comprising over 80,000 compounds. They not only exhibit important functions in plant physiology but also have commercial potential in the biotechnological, pharmaceutical, and agricultural sectors due to their promising properties, including various bioactivities against pathogens, inflammations, and cancer. In this work, we therefore aimed to implement the plant sesquiterpenoid pathway leading to β-caryophyllene in the heterologous host Rhodobacter capsulatus and achieved a maximum production of 139 ± 31 mg L−1 culture. As this sesquiterpene offers various beneficial anti-phytopathogenic activities, we evaluated the bioactivity of β-caryophyllene and its oxygenated derivative β-caryophyllene oxide against different phytopathogenic fungi. Here, both compounds significantly inhibited the growth of Sclerotinia sclerotiorum and Fusarium oxysporum by up to 40%, while growth of Alternaria brassicicola was only slightly affected, and Phoma lingam and Rhizoctonia solani were unaffected. At the same time, the compounds showed a promising low inhibitory profile for a variety of plant growth-promoting bacteria at suitable compound concentrations. Our observations thus give a first indication that β-caryophyllene and β-caryophyllene oxide are promising natural agents, which might be applicable for the management of certain plant pathogenic fungi in agricultural crop production.
LB  - PUB:(DE-HGF)16
C6  - 33466643
UR  - <Go to ISI:>//WOS:000610578900001
DO  - DOI:10.3390/microorganisms9010168
UR  - https://juser.fz-juelich.de/record/890513
ER  -