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@ARTICLE{Zhang:890796,
author = {Zhang, Weiyi and Luo, Cheng and Scossa, Federico and Zhang,
Qinghua and Usadel, Björn and Fernie, Alisdair R. and Mei,
Hanwei and Wen, Weiwei},
title = {{A} phased genome based on single sperm sequencing reveals
crossover pattern and complex relatedness in tea plants},
journal = {The plant journal},
volume = {105},
number = {1},
issn = {1365-313X},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2021-01204},
pages = {197 - 208},
year = {2021},
note = {Grant name:031B0779A (TEABAG - Ein pangenomischer Ansatz
zur Sicherung der Teeproduktion)031A536C (de.NBI -
Etablierungsphase: Leistungszentrum GCBN – Deutsches
BioGreenformatics-Netzwerk für Kulturpflanzen).},
abstract = {For diploid organisms that are highly heterozygous, a
phased haploid genome can greatly aid in functional genomic,
population genetic and breeding studies. Based on the genome
sequencing of 135 single sperm cells of the elite tea
cultivar ‘Fudingdabai’, we herein phased the genome of
Camellia sinensis, one of the most popular beverage crops
worldwide. High‐resolution genetic and recombination maps
of Fudingdabai were constructed, which revealed that
crossover (CO) positions were frequently located in the 5′
and 3′ ends of annotated genes, while CO distributions
across the genome were random. The low CO frequency in tea
can be explained by strong CO interference, and CO
simulation revealed the proportion of interference
insensitive CO ranged from $5.2\%$ to $11.7\%.$ We
furthermore developed a method to infer the relatedness
between tea accessions and detected complex kinship and
genetic signatures of 106 tea accessions. Among them, 59
accessions were closely related with Fudingdabai and 31 of
them were first‐degree relatives. We additionally
identified genes displaying allele specific expression
patterns between the two haplotypes of Fudingdabai and genes
displaying significantly differential expression levels
between Fudingdabai and other haplotypes. These results lay
the foundation for further investigation of genetic and
epigenetic factors underpinning the regulation of gene
expression and provide insights into the evolution of tea
plants as well as a valuable genetic resource for future
breeding efforts.},
cin = {IBG-4},
ddc = {580},
cid = {I:(DE-Juel1)IBG-4-20200403},
pnm = {217 - Für eine nachhaltige Bio-Ökonomie – von
Ressourcen zu Produkten (POF4-217)},
pid = {G:(DE-HGF)POF4-217},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33118252},
UT = {WOS:000596452300001},
doi = {10.1111/tpj.15051},
url = {https://juser.fz-juelich.de/record/890796},
}