TY  - JOUR
AU  - Tohge, Takayuki
AU  - Scossa, Federico
AU  - Wendenburg, Regina
AU  - Frasse, Pierre
AU  - Balbo, Ilse
AU  - Watanabe, Mutsumi
AU  - Alseekh, Saleh
AU  - Jadhav, Sagar Sudam
AU  - Delfin, Jay C.
AU  - Lohse, Marc
AU  - Giavalisco, Patrick
AU  - Usadel, Björn
AU  - Zhang, Youjun
AU  - Luo, Jie
AU  - Bouzayen, Mondher
AU  - Fernie, Alisdair R.
TI  - Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex
JO  - Molecular plant
VL  - 13
IS  - 7
SN  - 1674-2052
CY  - Oxford
PB  - Oxford Univ. Press
M1  - FZJ-2021-01211
SP  - 1027 - 1046
PY  - 2020
AB  - While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species, those defining specialized metabolism are less well characterized and more highly variable across species. In this study, we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of tomato. For this purpose, GC–MS- and LC–MS-based metabolomics of different tissues of Solanum lycopersicum and wild tomato species were carried out, in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis, and publicly available RNA-sequencing data for annotation of biosynthetic genes. The combined data were used to compile species-specific metabolic networks of polyphenolic metabolism, allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid pathway. The combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit ripening. Our results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.
LB  - PUB:(DE-HGF)16
C6  - 32305499
UR  - <Go to ISI:>//WOS:000547375600012
DO  - DOI:10.1016/j.molp.2020.04.004
UR  - https://juser.fz-juelich.de/record/890803
ER  -