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@ARTICLE{Tohge:890803,
author = {Tohge, Takayuki and Scossa, Federico and Wendenburg, Regina
and Frasse, Pierre and Balbo, Ilse and Watanabe, Mutsumi and
Alseekh, Saleh and Jadhav, Sagar Sudam and Delfin, Jay C.
and Lohse, Marc and Giavalisco, Patrick and Usadel, Björn
and Zhang, Youjun and Luo, Jie and Bouzayen, Mondher and
Fernie, Alisdair R.},
title = {{E}xploiting {N}atural {V}ariation in {T}omato to {D}efine
{P}athway {S}tructure and {M}etabolic {R}egulation of
{F}ruit {P}olyphenolics in the {L}ycopersicum {C}omplex},
journal = {Molecular plant},
volume = {13},
number = {7},
issn = {1674-2052},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {FZJ-2021-01211},
pages = {1027 - 1046},
year = {2020},
abstract = {While the structures of plant primary metabolic pathways
are generally well defined and highly conserved across
species, those defining specialized metabolism are less well
characterized and more highly variable across species. In
this study, we investigated polyphenolic metabolism in the
lycopersicum complex by characterizing the underlying
biosynthetic and decorative reactions that constitute the
metabolic network of polyphenols across eight different
species of tomato. For this purpose, GC–MS- and
LC–MS-based metabolomics of different tissues of Solanum
lycopersicum and wild tomato species were carried out, in
concert with the evaluation of cross-hybridized microarray
data for MapMan-based transcriptomic analysis, and publicly
available RNA-sequencing data for annotation of biosynthetic
genes. The combined data were used to compile
species-specific metabolic networks of polyphenolic
metabolism, allowing the establishment of an entire
pan-species biosynthetic framework as well as annotation of
the functions of decoration enzymes involved in the
formation of metabolic diversity of the flavonoid pathway.
The combined results are discussed in the context of the
current understanding of tomato flavonol biosynthesis as
well as a global view of metabolic shifts during fruit
ripening. Our results provide an example as to how
large-scale biology approaches can be used for the
definition and refinement of large specialized metabolism
pathways.},
cin = {IBG-4},
ddc = {580},
cid = {I:(DE-Juel1)IBG-4-20200403},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
typ = {PUB:(DE-HGF)16},
pubmed = {32305499},
UT = {WOS:000547375600012},
doi = {10.1016/j.molp.2020.04.004},
url = {https://juser.fz-juelich.de/record/890803},
}