000890936 001__ 890936
000890936 005__ 20230123101844.0
000890936 0247_ $$2doi$$a10.3390/ani11030710
000890936 0247_ $$2Handle$$a2128/31398
000890936 0247_ $$2pmid$$a33807941
000890936 0247_ $$2WOS$$aWOS:000633194800001
000890936 037__ $$aFZJ-2021-01258
000890936 082__ $$a590
000890936 1001_ $$0P:(DE-Juel1)131691$$aKroll, Tina$$b0$$eCorresponding author
000890936 245__ $$aAdditional Assessment of Fecal Corticosterone Metabolites Improves Visual Rating in the Evaluation of Stress Responses of Laboratory Rats
000890936 260__ $$aBasel$$bMDPI$$c2021
000890936 3367_ $$2DRIVER$$aarticle
000890936 3367_ $$2DataCite$$aOutput Types/Journal article
000890936 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1656492813_25194
000890936 3367_ $$2BibTeX$$aARTICLE
000890936 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000890936 3367_ $$00$$2EndNote$$aJournal Article
000890936 520__ $$aSince animal experiments cannot be completely avoided, the pain, suffering, and distress of laboratory animals must be minimized. To this end, a major prerequisite is reliable assessment of pain and distress. Usually, evaluation of animal welfare is done by visual inspection and score sheets. However, relatively little is known about whether standardized, but subjective, score sheets are able to reliably reflect the status of the animals. The current study aimed to compare visual assessment scores and changes in body weight with concentrations of fecal corticosterone metabolites (FCMs) in a neuroscientific experimental setup. Additionally, effects of refinement procedures were investigated. Eight male adult Sprague-Dawley rats underwent several experimental interventions, including electroencephalograph electrode implantation and subsequent recording, positron emission tomography (PET), and sleep deprivation (SD) by motorized activity wheels. Additional 16 rats were either used as controls without any treatment or to evaluate refinement strategies. Stress responses were determined on a daily basis by means of measuring FCMs, body weight, and evaluation of the animals’ welfare by standardized score sheets. Surgery provoked a significant elevation of FCM levels for up to five days. Increases in FCMs due to PET procedures or SD in activity wheels were also highly significant, while visual assessment scores did not indicate elevated stress levels and body weights remained constant. Visual assessment scores correlate with neither changes in body weight nor increases in FCM levels. Habituation procedures to activity wheels used for SD had no impact on corticosterone release. Our results revealed that actual score sheets for visual assessment of animal welfare did not mirror physiological stress responses assessed by FCM measurements. Moreover, small changes in body weight did not correlate with FCM concentration either. In conclusion, as visual assessment is a method allowing immediate interventions on suffering animals to alleviate burden, timely stress assessment in experimental rodents via score sheets should be ideally complemented by validated objective measures (e.g., fecal FCM measured by well-established assays for reliable detection of FCMs). This will complete a comprehensive appraisal of the animals’ welfare status in a retrospective manner and refine stressor procedures in the long run.
000890936 536__ $$0G:(DE-HGF)POF4-5253$$a5253 - Neuroimaging (POF4-525)$$cPOF4-525$$fPOF IV$$x0
000890936 588__ $$aDataset connected to CrossRef
000890936 7001_ $$0P:(DE-Juel1)131620$$aKornadt-Beck, Nikola$$b1$$ufzj
000890936 7001_ $$0P:(DE-Juel1)131712$$aOskamp, Angela$$b2$$ufzj
000890936 7001_ $$0P:(DE-Juel1)131679$$aElmenhorst, David$$b3$$ufzj
000890936 7001_ $$0P:(DE-HGF)0$$aTouma, Chadi$$b4
000890936 7001_ $$00000-0001-9466-3662$$aPalme, Rupert$$b5
000890936 7001_ $$0P:(DE-Juel1)131672$$aBauer, Andreas$$b6$$ufzj
000890936 773__ $$0PERI:(DE-600)2606558-7$$a10.3390/ani11030710$$gVol. 11, no. 3, p. 710 -$$n3$$p710 -$$tAnimals / Molecular Diversity Preservation International, MDPI$$v11$$x2076-2615$$y2021
000890936 8564_ $$uhttps://juser.fz-juelich.de/record/890936/files/Invoice_MDPI_animals-1094771_1530.00CHF.pdf
000890936 8564_ $$uhttps://juser.fz-juelich.de/record/890936/files/animals-11-00710.pdf$$yOpenAccess
000890936 8767_ $$8animals-1094771$$92021-03-02$$d2021-03-08$$eAPC$$jZahlung erfolgt$$panimals-1094771$$zCHF 1530,-, Belegnr. 1200164543 / 2021
000890936 909CO $$ooai:juser.fz-juelich.de:890936$$pdnbdelivery$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire
000890936 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131691$$aForschungszentrum Jülich$$b0$$kFZJ
000890936 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131620$$aForschungszentrum Jülich$$b1$$kFZJ
000890936 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131712$$aForschungszentrum Jülich$$b2$$kFZJ
000890936 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131679$$aForschungszentrum Jülich$$b3$$kFZJ
000890936 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131672$$aForschungszentrum Jülich$$b6$$kFZJ
000890936 9131_ $$0G:(DE-HGF)POF4-525$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5253$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vDecoding Brain Organization and Dysfunction$$x0
000890936 9141_ $$y2022
000890936 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-02-03
000890936 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000890936 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bANIMALS-BASEL : 2019$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000890936 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2021-02-03
000890936 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-02-03
000890936 920__ $$lyes
000890936 9201_ $$0I:(DE-Juel1)INM-2-20090406$$kINM-2$$lMolekulare Organisation des Gehirns$$x0
000890936 980__ $$ajournal
000890936 980__ $$aVDB
000890936 980__ $$aUNRESTRICTED
000890936 980__ $$aI:(DE-Juel1)INM-2-20090406
000890936 980__ $$aAPC
000890936 9801_ $$aAPC
000890936 9801_ $$aFullTexts