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@ARTICLE{Gerlach:890945,
      author       = {Gerlach, Tim and Nugroho, David Limanhadi and Rother,
                      Dörte},
      title        = {{T}he {E}ffect of {V}isible {L}ight on the {C}atalytic
                      {A}ctivity of {PLP}‐{D}ependent {E}nzymes},
      journal      = {ChemCatChem},
      volume       = {13},
      number       = {10},
      issn         = {1867-3880},
      address      = {Weinheim},
      publisher    = {WILEY-VCH Verlag},
      reportid     = {FZJ-2021-01267},
      pages        = {2398-2406},
      year         = {2021},
      note         = {This project was funded by the European Research Council in
                      frame of ERC starting grant 757320 „Light Controlled
                      Synthetic Enzyme Cascades”.},
      abstract     = {Pyridoxal 5’‐phosphate (PLP)‐dependent enzymes are a
                      versatile class of biocatalysts and feature a variety of
                      industrial applications. However, PLP is light sensitive and
                      can cause inactivation of enzymes in certain light
                      conditions. As most of the PLP‐dependent enzymes are
                      usually not handled in dark conditions, we evaluated the
                      effect of visible light on the activity of PLP‐dependent
                      enzymes during production as well as transformation. We
                      tested four amine transaminases, from Chromobacterium
                      violaceum, Bacillus megaterium, Vibrio fluvialis and a
                      variant from Arthrobacter species as well as two lysine
                      decarboxylases, from Selenomonas ruminantium and the LDCc
                      from Escherichia coli. It appeared that five of these six
                      enzymes suffered from a significant decrease in activity by
                      up to $90 \%$ when handled in laboratory light conditions.
                      Surprisingly, only the amine transaminase variant from
                      Arthrobacter species appeared to be unaffected by light
                      exposure and even showed an activation to $150 \%$
                      relative activity over the course of 6 h regardless of the
                      light conditions.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000634867100001},
      doi          = {10.1002/cctc.202100163},
      url          = {https://juser.fz-juelich.de/record/890945},
}