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@ARTICLE{Frimpong:890995,
      author       = {Frimpong, Felix and Windt, Carel W. and van Dusschoten,
                      Dagmar and Naz, Ali A. and Frei, Michael and Fiorani, Fabio},
      title        = {{A} {W}ild {A}llele of {P}yrroline-5-{C}arboxylate
                      {S}ynthase1 {L}eads to {P}roline {A}ccumulation in {S}pikes
                      and {L}eaves of {B}arley {C}ontributing to {I}mproved
                      {P}erformance {U}nder {R}educed {W}ater {A}vailability},
      journal      = {Frontiers in plant science},
      volume       = {12},
      issn         = {1664-462X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2021-01302},
      pages        = {633448},
      year         = {2021},
      abstract     = {Water stress (WS) during spike development strongly affects
                      final grain yield and grain quality in cereals. Proline, an
                      osmoprotectant amino-acid, may contribute to alleviating the
                      effects of cell and tissue dehydration. We studied five
                      spring barley genotypes contrasting in their drought
                      response, including two introgression lines, S42IL-143 and
                      S42IL-141, harboring a Pyrroline-5-carboxylate synthase1-
                      P5cs1 allele originating from the wild barley accession
                      ISR42-8. We tested the hypothesis that barley genotypes
                      harboring a wild allele at P5cs1 locus are comparatively
                      more drought-tolerant at the reproductive stage by inducing
                      proline accumulation in their immature spikes. At the
                      booting stage, we subjected plants to well-watered and WS
                      treatments until physiological maturity. Several
                      morpho-physiological traits had significant genotype by
                      treatment interaction and reduction under WS. Varying levels
                      of genotypic proline accumulation and differences in WS
                      tolerance were observed. Spike proline accumulation was
                      higher than leaf proline accumulation for all genotypes
                      under WS. Also, introgression lines carrying a wild allele
                      at P5cs1 locus had a markedly higher spike and leaf proline
                      content compared with the other genotypes. These
                      introgression lines showed milder drought symptoms compared
                      with elite genotypes, remained photosynthetically active
                      under WS, and maintained their intrinsic water use
                      efficiency. These combined responses contributed to the
                      achievement of higher final seed productivity. Magnetic
                      resonance imaging (MRI) of whole spikes at the soft dough
                      stage showed an increase in seed abortion among the elite
                      genotypes compared with the introgression lines 15 days
                      after WS treatment. Our results suggest that proline
                      accumulation at the reproductive stage contributes to the
                      maintenance of grain formation under water shortage.},
      cin          = {IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582) / 2171 - Biological and
                      environmental resources for sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF3-582 / G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33719307},
      UT           = {WOS:000627351100001},
      doi          = {10.3389/fpls.2021.633448},
      url          = {https://juser.fz-juelich.de/record/890995},
}