TY  - JOUR
AU  - Zhou, Chen
AU  - Bette, Sebastian
AU  - Babendreyer, Aaron
AU  - Hoffmann, Christina
AU  - Gerlach, Sven
AU  - Kremers, Tom
AU  - Ludwig, Andreas
AU  - Hoffmann, Bernd
AU  - Merkel, Rudolf
AU  - Uhlig, Stefan
AU  - Schnakenberg, Uwe
TI  - Stretchable electrical cell-substrate impedance sensor platform for monitoring cell monolayers under strain
JO  - Sensors and actuators  / B
VL  - 336
SN  - 0925-4005
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2021-01307
SP  - 129656 -
PY  - 2021
AB  - Stretchable microelectrodes paired with an ultra-elastic substrate can be used for electrical sensing of mechanically stretched cells and cell monolayers. Here, we present the development of a cell-stretching platform with thin-film interdigitated microelectrodes. Up to 35 % cyclic stretch are feasible with a novel interlaced meander design connected to the microelectrodes and using Poly(dimethylsiloxane) (PDMS) with a Young’s modulus of 50 kPa as an ultra-elastic substrate. Reliable electrical contacting of the microelectrodes under stretch was achieved by perforation of the contact pads. The novel platform enables label-free, real-time electrical cell-substrate impedance (ECIS) monitoring of cell monolayers. Proof-of-concept experiments indicated that electrical impedance of Madin-Darby canine kidney (MDCK) cell monolayers increased sharply by uniaxial mechanical strain above 20 %. For comparison, human alveolar basal epithelial adenocarcinoma (A549) cell monolayers, which are known to lack mature cell junctions, showed a continuous decrease of electrical impedance over the whole applied strain range of 35 %. The data reveal that impedance changes upon stretching depend on epithelial cell types and existence of tight cellular junctions. The system provides the basis for reliable continuous long-term monitoring of electrical properties of cell monolayers under strain by electrical impedance spectroscopy, e.g., to monitor epithelial permeability changes in real time and under label-free conditions to screen the influence of pharmacological substances.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000639153300007
DO  - DOI:10.1016/j.snb.2021.129656
UR  - https://juser.fz-juelich.de/record/891002
ER  -