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| 001 | 891002 | ||
| 005 | 20240705080645.0 | ||
| 024 | 7 | _ | |a 10.1016/j.snb.2021.129656 |2 doi |
| 024 | 7 | _ | |a 0925-4005 |2 ISSN |
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| 037 | _ | _ | |a FZJ-2021-01307 |
| 082 | _ | _ | |a 620 |
| 100 | 1 | _ | |a Zhou, Chen |0 P:(DE-HGF)0 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Stretchable electrical cell-substrate impedance sensor platform for monitoring cell monolayers under strain |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2021 |b Elsevier Science |
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| 520 | _ | _ | |a Stretchable microelectrodes paired with an ultra-elastic substrate can be used for electrical sensing of mechanically stretched cells and cell monolayers. Here, we present the development of a cell-stretching platform with thin-film interdigitated microelectrodes. Up to 35 % cyclic stretch are feasible with a novel interlaced meander design connected to the microelectrodes and using Poly(dimethylsiloxane) (PDMS) with a Young’s modulus of 50 kPa as an ultra-elastic substrate. Reliable electrical contacting of the microelectrodes under stretch was achieved by perforation of the contact pads. The novel platform enables label-free, real-time electrical cell-substrate impedance (ECIS) monitoring of cell monolayers. Proof-of-concept experiments indicated that electrical impedance of Madin-Darby canine kidney (MDCK) cell monolayers increased sharply by uniaxial mechanical strain above 20 %. For comparison, human alveolar basal epithelial adenocarcinoma (A549) cell monolayers, which are known to lack mature cell junctions, showed a continuous decrease of electrical impedance over the whole applied strain range of 35 %. The data reveal that impedance changes upon stretching depend on epithelial cell types and existence of tight cellular junctions. The system provides the basis for reliable continuous long-term monitoring of electrical properties of cell monolayers under strain by electrical impedance spectroscopy, e.g., to monitor epithelial permeability changes in real time and under label-free conditions to screen the influence of pharmacological substances. |
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| 773 | _ | _ | |a 10.1016/j.snb.2021.129656 |g Vol. 336, p. 129656 - |0 PERI:(DE-600)1500731-5 |p 129656 - |t Sensors and actuators |v 336 |y 2021 |x 0925-4005 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/891002/files/Stretchable%20electrical%20cell_umformatiert.pdf |y Published on 2021-02-13. Available in OpenAccess from 2023-02-13. |
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