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000891045 1001_ $$0P:(DE-Juel1)161196$$aMenzel, Miriam$$b0$$eCorresponding author
000891045 245__ $$aScattered Light Imaging: Resolving the substructure of nerve fiber crossings in whole brain sections with micrometer resolution
000891045 260__ $$aOrlando, Fla.$$bAcademic Press$$c2021
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000891045 520__ $$aFor developing a detailed network model of the brain based on image reconstructions, it is necessary to spatially resolve crossing nerve fibers. The accuracy hereby depends on many factors, including the spatial resolution of the imaging technique. 3D Polarized Light Imaging (3D-PLI) allows the three-dimensional reconstruction of nerve fiber tracts in whole brain sections with micrometer in-plane resolution, but leaves uncertainties in pixels containing crossing fibers. Here we introduce Scattered Light Imaging (SLI) to resolve the substructure of nerve fiber crossings. The measurement is performed on the same unstained histological brain sections as in 3D-PLI. By illuminating the brain sections from different angles and measuring the transmitted (scattered) light under normal incidence, light intensity profiles are obtained that are characteristic for the underlying brain tissue structure. We have developed a fully automated evaluation of the intensity profiles, allowing the user to extract various characteristics, like the individual directions of in-plane crossing nerve fibers, for each image pixel at once. We validate the reconstructed nerve fiber directions against results from previous simulation studies, scatterometry measurements, and fiber directions obtained from 3D-PLI. We demonstrate in different brain samples (human optic tracts, vervet monkey brain, rat brain) that the 2D fiber directions can be reliably reconstructed for up to three crossing nerve fiber bundles in each image pixel with an in-plane resolution of up to 6.5 $\mu$m. We show that SLI also yields reliable fiber directions in brain regions with low 3D-PLI signals coming from regions with a low density of myelinated nerve fibers or out-of-plane fibers. This makes Scattered Light Imaging a promising new imaging technique, providing crucial information about the organization of crossing nerve fibers in the brain.
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000891045 536__ $$0G:(DE-Juel1)jinm11_20191101$$a3D Reconstruction of Nerve Fibers in the Human, the Monkey, the Rodent, and the Pigeon Brain (jinm11_20191101)$$cjinm11_20191101$$f3D Reconstruction of Nerve Fibers in the Human, the Monkey, the Rodent, and the Pigeon Brain$$x4
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000891045 7001_ $$0P:(DE-Juel1)167509$$aReuter, Jan André$$b1
000891045 7001_ $$0P:(DE-Juel1)131642$$aGräßel, David$$b2
000891045 7001_ $$0P:(DE-Juel1)177812$$aHuwer, Mike$$b3
000891045 7001_ $$0P:(DE-Juel1)157671$$aSchlömer, Philipp$$b4
000891045 7001_ $$0P:(DE-Juel1)131631$$aAmunts, Katrin$$b5
000891045 7001_ $$0P:(DE-Juel1)131632$$aAxer, Markus$$b6
000891045 773__ $$0PERI:(DE-600)1471418-8$$a10.1016/j.neuroimage.2021.117952$$gVol. 233, p. 117952 -$$p117952 -$$tNeuroImage$$v233$$x1053-8119$$y2021
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