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@ARTICLE{Hnnefeld:891080,
      author       = {Hünnefeld, Max and Viets, Ulrike and Sharma, Vikas and
                      Wirtz, Astrid and Hardy, Aël and Frunzke, Julia},
      title        = {{G}enome {S}equence of the {B}acteriophage {CL}31 and
                      {I}nteraction with the {H}ost {S}train {C}orynebacterium
                      glutamicum {ATCC} 13032},
      journal      = {Viruses},
      volume       = {13},
      number       = {3},
      issn         = {1999-4915},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2021-01351},
      pages        = {495 -},
      year         = {2021},
      note         = {Biotechnologie 1},
      abstract     = {In this study, we provide a comprehensive analysis of the
                      genomic features of the phage CL31 and the infection
                      dynamics with the biotechnologically relevant host strain
                      Corynebacterium glutamicum ATCC 13032. Genome sequencing and
                      annotation of CL31 revealed a 45-kbp genome composed of 72
                      open reading frames, mimicking the GC content of its host
                      strain $(54.4\%).$ An ANI-based distance matrix showed the
                      highest similarity of CL31 to the temperate corynephage
                      Φ16. While the C. glutamicum ATCC 13032 wild type strain
                      showed only mild propagation of CL31, a strain lacking the
                      cglIR-cglIIR-cglIM restriction-modification system was
                      efficiently infected by this phage. Interestingly, the
                      prophage-free strain C. glutamicum MB001 featured an even
                      accelerated amplification of CL31 compared to the ∆resmod
                      strain suggesting a role of cryptic prophage elements in
                      phage defense. Proteome analysis of purified phage particles
                      and transcriptome analysis provide important insights into
                      structural components of the phage and the response of C.
                      glutamicum to CL31 infection. Isolation and sequencing of
                      CL31-resistant strains revealed SNPs in genes involved in
                      mycolic acid biosynthesis suggesting a role of this cell
                      envelope component in phage adsorption. Altogether, these
                      results provide an important basis for further investigation
                      of phage-host interactions in this important
                      biotechnological model organism.},
      cin          = {IBG-1},
      ddc          = {050},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33802915},
      UT           = {WOS:000634227800001},
      doi          = {10.3390/v13030495},
      url          = {https://juser.fz-juelich.de/record/891080},
}