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@PHDTHESIS{Hondrich:891147,
      author       = {Hondrich, Timm},
      title        = {{O}ptogenetic and electrical investigation of network
                      dynamics in patterned neuronal cultures},
      volume       = {68},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2021-01396},
      isbn         = {978-3-95806-555-0},
      series       = {Schriften des Forschungszentrums Jülich Reihe Information
                      / Information},
      pages        = {x, 177},
      year         = {2021},
      note         = {Dissertation, RWTH Aachen University, 2021},
      abstract     = {Our nervous system is one of the most complex systems on
                      earth. To investigate some ofthe nervous system’s basic
                      principles, neuronal cell cultures provide a highly
                      controllable,experimental platform of reduced complexity.
                      These basic principles include periods ofsynchronous
                      neuronal activity that can be an important mediator of
                      higher functions suchas memory. Another basic principle
                      governing the nervous system’s functionality is
                      itsmodularity. Anatomical modularity can be modeled in vitro
                      using neuronal patterningtechniques, one of which is
                      microcontact printing. The functional connectivity of such
                      patternednetworks was interrogated using optogenetic
                      techniques, such as calcium indicatorsand light-gated ion
                      channels, or electrophysiological methods, such as
                      patch-clamping ormicroelectrode arrays. In the first part of
                      this thesis, I modified different methods offeringcontrol
                      over neuronal cell cultures. The control over cellular
                      localization could beimproved by chemically uncoupling
                      substrate from coating via the silane GLYMO. Thisprevents
                      cells almost completely from growing on the cell-repellent
                      background instead ofthe cell-attractive pattern. Moreover,
                      microelectrode arrays with holey gold as a
                      conductivematerial were used for electrical recordings. With
                      its plasmonically induced, threefoldincrease in transparency
                      compared to solid gold, holey gold can be combined with
                      toolsfor controlling neurons optically, such as
                      optogenetics. In the second and third part of thisthesis, I
                      investigated the functional properties - such as signal
                      propagation, synchronicity,and network connectivity - of
                      modular patterned neuronal networks in an
                      all-opticalapproach. The triangular, anatomical modules
                      direct neuronal action potentials preferentiallytowards
                      their tip and subsequent modules. This is true for both main
                      designs,an elliptic one and one with a small upstream module
                      connected to a larger population.This directionality, and an
                      increased calcium response to same-module stimulations,
                      indicatesthat triangular anatomical modules also represent
                      functional modules.},
      cin          = {IBI-3},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:0001-2021080911},
      url          = {https://juser.fz-juelich.de/record/891147},
}