% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@PHDTHESIS{Nrbel:891635,
      author       = {Nörbel, Lena},
      title        = {{DNA}-capped silver nanoparticles for stochastic
                      nanoparticle impact electrochemisty},
      volume       = {66},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2021-01631},
      isbn         = {978-3-95806-541-3},
      series       = {Schriften des Forschungszentrums Jülich Reihe Information
                      / Information},
      pages        = {VI, 142},
      year         = {2021},
      note         = {Dissertation, RWTH Aachen University, 2021},
      abstract     = {One of the major challenges in analytical chemistry is
                      reducing the detection limit of an analytedown to a level
                      where the specific identification of a single entity is
                      possible. In thiscontext, nano-impact electrochemistry is
                      one of the most active and promising research areasin the
                      field of single-entity experiments. This method is a
                      versatile analytical procedurefor characterization and
                      real-time monitoring of bioconjugation and biomolecular
                      recognitionevents as well as for ultrasensitive detection of
                      a variety of biological species. The combinationof a highly
                      sensitive amplifier system and high-density microelectrode
                      arrays allows detectionof single silver nanoparticle impacts
                      down to subpicomolar concentrations. For the
                      analytedetection, silver nanoparticles are modied with
                      biomolecular receptors alternating their impactfrequency on
                      the electrode surface. Thus, the particles serve as redox
                      tags convertingan otherwise redox-inactive target into an
                      electrochemically detectable species. In this work,silver
                      nanoparticles were modied with thiolated single stranded
                      oligonucleotides with varyingmolar ratios of DNA to
                      nanoparticles. The modified conjugation protocol resulted in
                      stableDNA-nanoparticle conjugates. In depth characterization
                      of these conjugates gave insight intotheir structural and
                      physicochemical properties. In a next step, the impact
                      behaviour of DNAcappednanoparticles was evaluated and
                      compared to citrate-capped nanoparticles.
                      Differentparameters were identified to inuence the impact
                      probability. First, the surface modicationresults in a
                      higher nanoparticle stability by preventing particle
                      aggregation, which increasesthe impact frequency, especially
                      in the presence of high salt concentrations. Second, the
                      redoxactivity is reduced in comparison to citrate stabilized
                      particles. In particular, the ligand surfacedensity as well
                      as the conformation and size of the receptor molecule were
                      found to play acrucial role. Furthermore, the composition of
                      the electrolyte and the applied potential affectthe impact
                      probability, but to a different extent as for citrate
                      stabilized particles. By carefullyadjusting the surface
                      density of ligands, a high particle stability is achieved
                      while maintainingtheir desired redox activity. The results
                      demonstrate that DNA-AgNPs possess impactcharacteristics
                      different from standard citrate stabilized particles. In a
                      last step, stochasticnanoparticle impact electrochemistry
                      was probed for the detection of DNA hybridizationevents on
                      the nanoparticle surface. The results disclose decreased
                      hybridization eficiencies onthe nanoparticle surface and
                      reveal that a surface-bound process is more complicated
                      whencompared to hybridization in solution.},
      cin          = {IBI-3},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {524 - Molecular and Cellular Information Processing
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-524},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:0001-2021052727},
      url          = {https://juser.fz-juelich.de/record/891635},
}