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@ARTICLE{RivasAiello:891693,
author = {Rivas Aiello, María Belén and Azcárate, Julio C. and
Zelaya, Eugenia and David Gara, Pedro and Bosio, Gabriela N.
and Gensch, Thomas and Mártire, Daniel O.},
title = {{P}hotothermal therapy with silver nanoplates in {H}e{L}a
cells studied by in situ fluorescence microscopy},
journal = {Biomaterials science},
volume = {9},
number = {7},
issn = {2047-4849},
address = {Cambridge},
publisher = {Royal Soc. of Chemistry},
reportid = {FZJ-2021-01674},
pages = {2608 - 2619},
year = {2021},
abstract = {Photothermal therapy (PTT) is a noninvasive treatment for
cancer relying on the incorporation of NIR-light absorbing
nanomaterials into cells, which upon illumination release
heat causing thermally induced cell death. We prove that
irradiation of aqueous suspensions of
poly(vinylpyrrolidone)-coated silver nanoplates (PVPAgNP) or
PVPAgNP in HeLa cells with red or NIR lasers causes a
sizeable photothermal effect, which in cells can be
visualized with the temperature sensing fluorophore
Rhodamine B (RhB) using spinning disk confocal fluorescence
microscopy or fluorescence lifetime imaging. Upon red-light
irradiation of cells that were incubated with both, RhB and
PVPAgNP at concentrations with no adverse effects on cell
viability, a substantial heat release is detected.
Initiation of cell death by photothermal effect is observed
by positive signals of fluorescent markers for early and
late apoptosis. Surprisingly, a new nanomaterial-assisted
cell killing mode is operating when PVPAgNP-loaded HeLa
cells are excited with moderate powers of fs-pulsed NIR
light. Small roundish areas are generated with bright and
fast (<1 ns) decaying emission, which expand fast and
destroy the whole cell in seconds. This characteristic
emission is assigned to efficient optical breakdown
initiation around the strongly absorbing PVPAgNP leading to
plasma formation that spreads fast through the cell.},
cin = {IBI-1},
ddc = {540},
cid = {I:(DE-Juel1)IBI-1-20200312},
pnm = {524 - Molecular and Cellular Information Processing
(POF4-524)},
pid = {G:(DE-HGF)POF4-524},
typ = {PUB:(DE-HGF)16},
pubmed = {33595000},
UT = {WOS:000635690000001},
doi = {10.1039/D0BM01952F},
url = {https://juser.fz-juelich.de/record/891693},
}