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000891697 0247_ $$2doi$$a10.1021/acsabm.0c01424
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000891697 041__ $$aEnglish
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000891697 1001_ $$0P:(DE-HGF)0$$aRuks, Tatjana$$b0
000891697 245__ $$aPeptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting
000891697 260__ $$aWashington, DC$$bACS Publications$$c2021
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000891697 520__ $$aUltrasmall gold nanoparticles with a metallic core diameter of 2 nm were surface-conjugated with peptides that selectively target epitopes on the surface of the WW domain of the model protein hPin1 (hPin1-WW). The binding to the gold surface was accomplished via the thiol group of a terminal cysteine. The particles were analyzed by NMR spectroscopy, high-resolution transmission electron microscopy, and differential centrifugal sedimentation. The surface loading was determined by conjugating a FAM-labeled peptide, followed by UV–vis spectroscopy, and by quantitative 1H NMR spectroscopy, showing about 150 peptide molecules conjugated to each nanoparticle. The interaction between the peptide-decorated nanoparticles with hPin1-WW was probed by 1H–15N-HSQC NMR titration, fluorescence polarization spectroscopy (FP), and isothermal titration calorimetry (ITC). The particles showed a similar binding (KD = 10–20 μM) compared to the dissolved peptides (KD = 10–30 μM). Small-angle X-ray scattering (SAXS) showed that the particles were well dispersed and did not agglomerate after the addition of hPin1-WW (no cross-linking by the protein). Each nanoparticle was able to bind about 20 hPin1-WW protein molecules. An unspecific interaction with hPin1 was excluded by the attachment of a nonbinding peptide to the nanoparticle surface. The uptake by cells was studied by confocal laser scanning microscopy. The peptide-functionalized nanoparticles penetrated the cell membrane and were located in the cytosol. In contrast, the dissolved peptide did not cross the cell membrane. Peptide-functionalized nanoparticles are promising agents to target proteins inside cells.
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000891697 7001_ $$0P:(DE-HGF)0$$aLoza, Kateryna$$b1
000891697 7001_ $$0P:(DE-Juel1)130695$$aHeggen, Marc$$b2
000891697 7001_ $$0P:(DE-HGF)0$$aPrymak, Oleg$$b3
000891697 7001_ $$00000-0002-3544-2277$$aSehnem, Andre Luiz$$b4
000891697 7001_ $$00000-0002-3426-6507$$aOliveira, Cristiano L. P.$$b5
000891697 7001_ $$0P:(DE-HGF)0$$aBayer, Peter$$b6
000891697 7001_ $$0P:(DE-HGF)0$$aBeuck, Christine$$b7$$eCorresponding author
000891697 7001_ $$00000-0002-1641-7068$$aEpple, Matthias$$b8$$eCorresponding author
000891697 773__ $$0PERI:(DE-600)2936886-8$$a10.1021/acsabm.0c01424$$gVol. 4, no. 1, p. 945 - 965$$n1$$p945 - 965$$tACS applied bio materials$$v4$$x2576-6422$$y2021
000891697 8564_ $$uhttps://juser.fz-juelich.de/record/891697/files/acsabm.0c01424.pdf
000891697 8564_ $$uhttps://juser.fz-juelich.de/record/891697/files/Peptide-Conjugated%20Ultrasmall%20Gold%20Nanoparticles.pdf$$yRestricted
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