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| Hauptseite > Publikationsdatenbank > Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting > print |
| Hauptseite > Publikationsdatenbank > Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting > print |
| 001 | 891697 | ||
| 005 | 20210623133416.0 | ||
| 024 | 7 | _ | |a 10.1021/acsabm.0c01424 |2 doi |
| 024 | 7 | _ | |a WOS:000643599900072 |2 WOS |
| 037 | _ | _ | |a FZJ-2021-01676 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Ruks, Tatjana |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting |
| 260 | _ | _ | |a Washington, DC |c 2021 |b ACS Publications |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1618314578_3239 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Ultrasmall gold nanoparticles with a metallic core diameter of 2 nm were surface-conjugated with peptides that selectively target epitopes on the surface of the WW domain of the model protein hPin1 (hPin1-WW). The binding to the gold surface was accomplished via the thiol group of a terminal cysteine. The particles were analyzed by NMR spectroscopy, high-resolution transmission electron microscopy, and differential centrifugal sedimentation. The surface loading was determined by conjugating a FAM-labeled peptide, followed by UV–vis spectroscopy, and by quantitative 1H NMR spectroscopy, showing about 150 peptide molecules conjugated to each nanoparticle. The interaction between the peptide-decorated nanoparticles with hPin1-WW was probed by 1H–15N-HSQC NMR titration, fluorescence polarization spectroscopy (FP), and isothermal titration calorimetry (ITC). The particles showed a similar binding (KD = 10–20 μM) compared to the dissolved peptides (KD = 10–30 μM). Small-angle X-ray scattering (SAXS) showed that the particles were well dispersed and did not agglomerate after the addition of hPin1-WW (no cross-linking by the protein). Each nanoparticle was able to bind about 20 hPin1-WW protein molecules. An unspecific interaction with hPin1 was excluded by the attachment of a nonbinding peptide to the nanoparticle surface. The uptake by cells was studied by confocal laser scanning microscopy. The peptide-functionalized nanoparticles penetrated the cell membrane and were located in the cytosol. In contrast, the dissolved peptide did not cross the cell membrane. Peptide-functionalized nanoparticles are promising agents to target proteins inside cells. |
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| 700 | 1 | _ | |a Loza, Kateryna |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Heggen, Marc |0 P:(DE-Juel1)130695 |b 2 |
| 700 | 1 | _ | |a Prymak, Oleg |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Sehnem, Andre Luiz |0 0000-0002-3544-2277 |b 4 |
| 700 | 1 | _ | |a Oliveira, Cristiano L. P. |0 0000-0002-3426-6507 |b 5 |
| 700 | 1 | _ | |a Bayer, Peter |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Beuck, Christine |0 P:(DE-HGF)0 |b 7 |e Corresponding author |
| 700 | 1 | _ | |a Epple, Matthias |0 0000-0002-1641-7068 |b 8 |e Corresponding author |
| 773 | _ | _ | |a 10.1021/acsabm.0c01424 |g Vol. 4, no. 1, p. 945 - 965 |0 PERI:(DE-600)2936886-8 |n 1 |p 945 - 965 |t ACS applied bio materials |v 4 |y 2021 |x 2576-6422 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/891697/files/acsabm.0c01424.pdf |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/891697/files/Peptide-Conjugated%20Ultrasmall%20Gold%20Nanoparticles.pdf |y Restricted |
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