TY  - JOUR
AU  - Casillo, Angela
AU  - Fabozzi, Antonio
AU  - Russo Krauss, Irene
AU  - Parrilli, Ermenegilda
AU  - Biggs, Caroline I.
AU  - Gibson, Matthew I.
AU  - Lanzetta, Rosa
AU  - Appavou, Marie-Sousai
AU  - Radulescu, Aurel
AU  - Tutino, Maria L.
AU  - Paduano, Luigi
AU  - Corsaro, Maria M.
TI  - Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides
JO  - Biomacromolecules
VL  - 22
SN  - 1526-4602
CY  - Columbus, Ohio
PB  - American Chemical Soc.
M1  - FZJ-2021-01723
SP  - 1445-1457
PY  - 2021
AB  - Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.
LB  - PUB:(DE-HGF)16
C6  - 33729771
UR  - <Go to ISI:>//WOS:000640310700010
DO  - DOI:10.1021/acs.biomac.0c01659
UR  - https://juser.fz-juelich.de/record/891765
ER  -