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@ARTICLE{Casillo:891765,
      author       = {Casillo, Angela and Fabozzi, Antonio and Russo Krauss,
                      Irene and Parrilli, Ermenegilda and Biggs, Caroline I. and
                      Gibson, Matthew I. and Lanzetta, Rosa and Appavou,
                      Marie-Sousai and Radulescu, Aurel and Tutino, Maria L. and
                      Paduano, Luigi and Corsaro, Maria M.},
      title        = {{P}hysicochemical {A}pproach to {U}nderstanding the
                      {S}tructure, {C}onformation, and {A}ctivity of {M}annan
                      {P}olysaccharides},
      journal      = {Biomacromolecules},
      volume       = {22},
      issn         = {1526-4602},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Soc.},
      reportid     = {FZJ-2021-01723},
      pages        = {1445-1457},
      year         = {2021},
      abstract     = {Extracellular polysaccharides are widely produced by
                      bacteria, yeasts, and algae. These polymers are involved in
                      several biological functions, such as bacteria adhesion to
                      surface and biofilm formation, ion sequestering, protection
                      from desiccation, and cryoprotection. The chemical
                      characterization of these polymers is the starting point for
                      obtaining relationships between their structures and their
                      various functions. While this fundamental correlation is
                      well reported and studied for the proteins, for the
                      polysaccharides, this relationship is less intuitive. In
                      this paper, we elucidate the chemical structure and
                      conformational studies of a mannan exopolysaccharide from
                      the permafrost isolated bacterium Psychrobacter arcticus
                      strain 273-4. The mannan from the cold-adapted bacterium was
                      compared with its dephosphorylated derivative and the
                      commercial product from Saccharomyces cerevisiae. Starting
                      from the chemical structure, we explored a new approach to
                      deepen the study of the structure/activity relationship. A
                      pool of physicochemical techniques, ranging from small-angle
                      neutron scattering (SANS) and dynamic and static light
                      scattering (DLS and SLS, respectively) to circular dichroism
                      (CD) and cryo-transmission electron microscopy (cryo-TEM),
                      have been used. Finally, the ice recrystallization
                      inhibition activity of the polysaccharides was explored. The
                      experimental evidence suggests that the mannan
                      exopolysaccharide from P. arcticus bacterium has an
                      efficient interaction with the water molecules, and it is
                      structurally characterized by rigid-rod regions assuming a
                      14-helix-type conformation.},
      cin          = {JCNS-FRM-II / MLZ / JCNS-1 / JCNS-4},
      ddc          = {570},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 / I:(DE-588b)4597118-3 /
                      I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-4-20201012},
      pnm          = {6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ)
                      (POF4-6G4) / 632 - Materials – Quantum, Complex and
                      Functional Materials (POF4-632)},
      pid          = {G:(DE-HGF)POF4-6G4 / G:(DE-HGF)POF4-632},
      experiment   = {EXP:(DE-MLZ)KWS2-20140101 / EXP:(DE-MLZ)TEM-MLZ-20151210},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33729771},
      UT           = {WOS:000640310700010},
      doi          = {10.1021/acs.biomac.0c01659},
      url          = {https://juser.fz-juelich.de/record/891765},
}