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@ARTICLE{vanSchie:892291,
      author       = {van Schie, Morten M. C. H. and Spöring, Jan-Dirk and
                      Bocola, Marco and Domínguez de María, Pablo and Rother,
                      Dörte},
      title        = {{A}pplied biocatalysis beyond just buffers – from aqueous
                      to unconventional media. {O}ptions and guidelines},
      journal      = {Green chemistry},
      volume       = {23},
      issn         = {1463-9270},
      address      = {Cambridge},
      publisher    = {RSC},
      reportid     = {FZJ-2021-02000},
      pages        = {3191-3206},
      year         = {2021},
      abstract     = {In nature, enzymes conventionally operate under aqueous
                      conditions. Because of this, aqueous buffers are often the
                      choice for reaction media when enzymes are applied in
                      chemical synthesis. However, to meet the demands of an
                      industrial application, due to the poor water solubility of
                      many industrially relevant compounds, an aqueous reaction
                      system will often not be able to provide sufficient
                      substrate loadings. A switch to a non-aqueous solvent system
                      can provide a solution, which is already common for lipases,
                      but more challenging for biocatalysts from other enzyme
                      classes. The choices in solvent types and systems, however,
                      can be overwhelming. Furthermore, some engineering of the
                      protein structure of biocatalyst formulation is required. In
                      this review, a guide for those working with biocatalysts,
                      who look for a way to increase their reaction productivity,
                      is presented. Examples reported clearly show that bulk water
                      is not necessarily required for biocatalytic reactions and
                      that clever solvent systems design can support increased
                      product concentrations thereby decreasing waste formation.
                      Additionally, under these conditions, enzymes can also be
                      combined in cascades with other, water-sensitive, chemical
                      catalysts. Finally, we show that the application of
                      non-aqueous solvents in biocatalysis can actually lead to
                      more sustainable processes. At the hand of flowcharts,
                      following simple questions, one can quickly find what
                      solvent systems are viable},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34093084},
      UT           = {WOS:000641283600001},
      doi          = {10.1039/D1GC00561H},
      url          = {https://juser.fz-juelich.de/record/892291},
}