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@ARTICLE{Costantini:892370,
      author       = {Costantini, Irene and Baria, Enrico and Sorelli, Michele
                      and Matuschke, Felix and Giardini, Francesco and Menzel,
                      Miriam and Mazzamuto, Giacomo and Silvestri, Ludovico and
                      Cicchi, Riccardo and Amunts, Katrin and Axer, Markus and
                      Pavone, Francesco Saverio},
      title        = {{A}utofluorescence enhancement for label-free imaging of
                      myelinated fibers in mammalian brains},
      journal      = {Scientific reports},
      volume       = {11},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {FZJ-2021-02031},
      pages        = {8038},
      year         = {2021},
      abstract     = {Analyzing the structure of neuronal fibers with single axon
                      resolution in large volumes is a challenge in connectomics.
                      Different technologies try to address this goal; however,
                      they are limited either by the ineffective labeling of the
                      fibers or in the achievable resolution. The possibility of
                      discriminating between different adjacent myelinated axons
                      gives the opportunity of providing more information about
                      the fiber composition and architecture within a specific
                      area. Here, we propose MAGIC (Myelin Autofluorescence
                      imaging by Glycerol Induced Contrast enhancement), a tissue
                      preparation method to perform label-free fluorescence
                      imaging of myelinated fibers that is user friendly and easy
                      to handle. We exploit the high axial and radial resolution
                      of two-photon fluorescence microscopy (TPFM) optical
                      sectioning to decipher the mixture of various fiber
                      orientations within the sample of interest. We demonstrate
                      its broad applicability by performing mesoscopic
                      reconstruction at a sub-micron resolution of mouse, rat,
                      monkey, and human brain samples and by quantifying the
                      different fiber organization in control and Reeler mouse's
                      hippocampal sections. Our study provides a novel method for
                      3D label-free imaging of nerve fibers in fixed samples at
                      high resolution, below micrometer level, that overcomes the
                      limitation related to the myelinated axons exogenous
                      labeling, improving the possibility of analyzing brain
                      connectivity.},
      cin          = {INM-1},
      ddc          = {600},
      cid          = {I:(DE-Juel1)INM-1-20090406},
      pnm          = {525 - Decoding Brain Organization and Dysfunction
                      (POF4-525) / HBP SGA3 - Human Brain Project Specific Grant
                      Agreement 3 (945539) / HBP SGA2 - Human Brain Project
                      Specific Grant Agreement 2 (785907)},
      pid          = {G:(DE-HGF)POF4-525 / G:(EU-Grant)945539 /
                      G:(EU-Grant)785907},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33850168},
      UT           = {WOS:000640611400023},
      doi          = {10.1038/s41598-021-86092-7},
      url          = {https://juser.fz-juelich.de/record/892370},
}