TY  - JOUR
AU  - Tiso, Till
AU  - Narancic, Tanja
AU  - Wei, Ren
AU  - Pollet, Eric
AU  - Beagan, Niall
AU  - Schröder, Katja
AU  - Honak, Annett
AU  - Jiang, Mengying
AU  - Kenny, Shane T.
AU  - Wierckx, Nick
AU  - Perrin, Rémi
AU  - Avérous, Luc
AU  - Zimmermann, Wolfgang
AU  - O'Connor, Kevin
AU  - Blank, Lars M.
TI  - Towards bio-upcycling of polyethylene terephthalate
JO  - Metabolic engineering
VL  - 66
SN  - 1096-7176
CY  - Orlando, Fla.
PB  - Academic Press
M1  - FZJ-2021-02052
SP  - 167 - 178
PY  - 2021
N1  - Biotechnologie 1
AB  - Over 359 million tons of plastics were produced worldwide in 2018, with significant growth expected in the near future, resulting in the global challenge of end-of-life management. The recent identification of enzymes that degrade plastics previously considered non-biodegradable opens up opportunities to steer the plastic recycling industry into the realm of biotechnology.Here, the sequential conversion of post-consumer polyethylene terephthalate (PET) into two types of bioplastics is presented: a medium chain-length polyhydroxyalkanoate (PHA) and a novel bio-based poly(amide urethane) (bio-PU). PET films are hydrolyzed by a thermostable polyester hydrolase yielding highly pure terephthalate and ethylene glycol. The obtained hydrolysate is used directly as a feedstock for a terephthalate-degrading Pseudomonas umsongensis GO16, also evolved to efficiently metabolize ethylene glycol, to produce PHA. The strain is further modified to secrete hydroxyalkanoyloxy-alkanoates (HAAs), which are used as monomers for the chemo-catalytic synthesis of bio-PU. In short, a novel value-chain for PET upcycling is shown that circumvents the costly purification of PET monomers, adding technological flexibility to the global challenge of end-of-life management of plastics.
LB  - PUB:(DE-HGF)16
C6  - 33865980
UR  - <Go to ISI:>//WOS:000658808700005
DO  - DOI:10.1016/j.ymben.2021.03.011
UR  - https://juser.fz-juelich.de/record/892391
ER  -